LF (N-Term) antibody

Antigen: LF (N-Term)

Clonality: Monoclonal (LFA27)

Host: Mouse

Reactivity: Bacteria

Application: Western Blotting (WB), ELISA

Catalog no.: ABIN305108

Additional Information

Swiss-Prot: P15917

Immunogen: Highly purified Bacillus anthracis lethal factor.

Isotype: IgG2a  (Matching secondary antibodies)

Clone: LFA27

Description: Gene: LF, Standard Gene Symbol: LF, Family: Toxin, Synonyms: LF

Specificity: Recognizes the Lethal Factor of the anthrax toxin. Bacillus anthracis produces a toxin composed of three proteins, the Protective Antigen (PA), the Edema Factor (EF) and the Lethal Factor (LF). The three proteins depend on each other for their toxic effect. Both EF and LF functions intracellularly but are too large to enter the cell through existing channels. The PA is responsible for transporting the EF and LF into cells. The LF acts as an endoprotease, removing the N-terminus of mitogen-activated protein kinase kinases (MAPKK). The kinases are prevented from binding to their substrates, which leads to altered signalling pathways and ultimately to apoptosis. May be used to detect the Lethal Factor of Bacillus anthracis in ELISA assays

Application Details

Application Notes: ELISA, WB

Purification: Affinity Chromatography

Buffer: PBS, pH 7.4, 0.09% sodium azide.

Storage: Long term: -20°C, Short term: -20°C

Restrictions: For Research Use only