B-Cells antibody (FITC)

Catalog No. ABIN305565

Product Details

Target: B-Cells

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: FITC

Application: Flow Cytometry (FACS)

Specificity: Recognizes a glycoprotein antigen of 105kD expressed by B lymphocytes. The FMC7 antigen is expressed by peripheral B lymphocytes, and has been used widely in the study of B cell malignancy. FMC7 has been used extensively to differentiate various types of B cell malignancy. B-CLL is generally considered to be negative for FMC7 expression, but strong staining is seen in many other types of B cell lymphoma, including prolymphocytic leukemia and hairy cell leukemia. The nature of the FMC7 antigen has remained poorly defined following its first description in 1981. The expression pattern closely corresponds to that seen with CD22, but studies have shown that FMC7 does not recognize the CD22 Molecule itself. A recent publication by Serke et al has shown Recognizes a conformational epitope on the CD20 Molecule, most likely a multimeric complex of CD20.

Purification: Gel filtration

Immunogen: HRIK cells - Human B-Lymphoblastoid line.

Type of Immunogen: Cells

Clone: FMC7

Isotype: IgM

Application Details

Application Notes: Approved: Flo (1:1)

Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells or 100 μL whole blood. Method sheets are available on request. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-HCL, 0.5 mL sodium chloride, 10 mM glycine,  pH 8.0, 0.09 % sodium azide, 1 % BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details

Target: B-Cells

Alternative Name: B Cell