Heat Shock 105kDa/110kDa Protein 1 (HSPH1) antibody

Antigen: Heat Shock 105kDa/110kDa Protein 1 (HSPH1)

Synonyms: hsph1, hsp105, MGC75864, hsph1b, MGC80255

Clonality: Monoclonal (J1G12)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN306515

Additional Information

Alternative name: HSP105?

Immunogen: Recombinant human HSP105 a (1-858 aa) purified from E. coli. Anti-human HSP105 a mAb, clone J1G12, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human HSP105 a protein.

Isotype: IgG2b kappa  (Matching secondary antibodies)

Clone: J1G12

Description: Hsp105 is a mammalian member of the HSP105/110 family, a diverged subgroup of the HSP70 family. HSP105 exists as two isoforms, alpha and beta. Hsp105 alpha associates with Hsp70/Hsc70 as complexes in vivo and regulates the chaperone activity of Hsp70/Hsc70 negatively in vitro and in vivo. Hsp105 a is constitutively expressed and also induced by various stress, whereas Hsp105 beta, lacking 44 amino acids from Hsp105 a, is specifically expressed during mild heat shock. Although Hsp105 a is shown to localize in the cytoplasm of mammalian cells, cellular localization of Hsp105 beta is not known

Application Details

Application Notes: The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for is 1:500 ~ 1,000.Recommended starting dilution is 1:1,000.

Buffer: Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide. Storage: Can be stored at 4

Restrictions: For Research Use only

Images

Western blot analysis: Cell lysates of HeLa(30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human HSP105 ? (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.