tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, beta Polypeptide (YWHAB) antibody

Antigen: tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, beta Polypeptide (YWHAB)

Synonyms: HS1, GW128, KCIP-1, 1300003C17Rik, ywhab, MGC53093, MGC78918, 14-3-3beta, hs1, gw128, kcip-1, YWHAB, 1433b

Clonality: Monoclonal (J2E9)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN306522

Additional Information

Alternative name: 14-3-3 beta

Immunogen: Recombinant human 14-3-3 beta (1-246aa) purified from E. coli. Anti-human 14-3-3 beta mAb, clone J2E9, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human YWHAB protein.

Isotype: IgG2a-kappa  (Matching secondary antibodies)

Clone: J2E9

Description: The 14-3-3 family plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon, sigma, theta, tau and eta that have been identified in mammals. The 14-3-3 beta, a subtype of the 14-3-3 proteins, was found in B Cells, brain and liver etc. This 14-3-3 beta has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery.

Application Details

Application Notes: The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for is 1:500 ~ 1,000.Recommended starting dilution is 1:1,000.

Concentration: 1mg/ml

Buffer: Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide. Storage: Can be stored at 4

Research Area: Transcription Factors

Restrictions: For Research Use only