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Antibodies
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anti-Nanog Homeobox Pseudogene 8 (NANOGP8) antibody
Nanog Homeobox Pseudogene 8 (NANOGP8) antibody
| Antigen |
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| Synonyms |
ENK, ecat4, 2410002E02Rik, PN8, NANOG, NANOGP1, MGC119250, nanog |
| Clonality |
Monoclonal (5A10) |
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Host
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Reactivity
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Human (109), Mouse (Murine) (31), Rat (Rattus) (24), Dog (Canine) (5), Chicken (3), Cow (Bovine) (3), Rabbit (2), Zebrafish (Brachydanio rerio) (2), Goat (1), Horse (Equine) (1), Pig (Porcine) (1), Sheep (Ovine) (1)
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Application
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Western Blotting (WB) (79), Immunofluorescence (IF) (51), Flow Cytometry (FACS) (37), ELISA (36), Enzyme Immunoassay (EIA) (25), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (18), Immunohistochemistry (IHC) (8), Immunoprecipitation (IP) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Dot Blot (Dot) (2), Immunocytochemistry (ICC) (2), Immunoelectron Microscopy (IEM) (2), Blocking Antibody (Inhibition) (1), ELISA (Detection) (1)
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| Catalog no. |
ABIN306525 |
| Quantity |
50ul (1 mg/ml) |
| Price |
300.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 to 7 Business Days |
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Alternative name
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Nanog
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Immunogen
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Recombinant human Nanog (1-154aa) purified from E. coli. Anti-human Nanog mAb, clone 5A10, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Nanog protein.
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Isotype
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IgG2a kappa (Matching secondary antibodies)
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Clone
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5A10
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Description
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Nanog is a multidomain homeobox transcription factor that functions to maintain the undifferentiated state of pluripotent stem cells. Nanog expression counteracts the differentiation-promoting signals induced by the extrinsic factors LIF, Stat3 and BMP. When Nanog expression is downregulated, cell differentiation can proceed. Proteins that regulate Nanog expression include transcription factors Oct4, SOX2, FoxD3, and Tcf3 and tumor suppressor p53.
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Application Notes
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The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for is 1:500 ~ 1:2000.Recommended starting dilution is 1:500.
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Concentration
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1 mg/ml
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Purification
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The antibody was purified from mouse ascitic fluids by protein-G affinity chromatography. Can be stored at 4C for up to one month, but store at -20C for long term storage. Avoid repeated freezing and thawing cycles.
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Buffer
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Liquid in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide
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Restrictions
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For Research Use only
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Alternatives for antigen "Nanog Homeobox Pseudogene 8 (NANOGP8)", type "Antibodies"
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Hosts
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Rabbit (84), Mouse (23), Goat (8)
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Reactivities
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Human (109), Mouse (Murine) (31), Rat (Rattus) (24), Dog (Canine) (5), Chicken (3), Cow (Bovine) (3), Rabbit (2), Zebrafish (Brachydanio rerio) (2), Goat (1), Horse (Equine) (1), Pig (Porcine) (1), Sheep (Ovine) (1)
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Applications
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Western Blotting (WB) (79), Immunofluorescence (IF) (51), Flow Cytometry (FACS) (37), ELISA (36), Enzyme Immunoassay (EIA) (25), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (18), Immunohistochemistry (IHC) (8), Immunoprecipitation (IP) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Dot Blot (Dot) (2), Immunocytochemistry (ICC) (2), Immunoelectron Microscopy (IEM) (2), Blocking Antibody (Inhibition) (1), ELISA (Detection) (1)
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Conjugates
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Biotin (7), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2)
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Epitopes
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C-Term (3), Center (3), N-Term (2), S285 (2), S71 (1)
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Alternatives 
