Internexin Neuronal Intermediate Filament Protein, alpha (INA) antibody

Antigen: Internexin Neuronal Intermediate Filament Protein, alpha (INA)

Synonyms: NEF5, NF-66, TXBP-1, FLJ18662, FLJ57501, MGC12702, Nf66, Inexa, Intlaa, NF66, AV028420, MGC25352, Ina, cb126, sb:cb126, ina-A, xefiltin, ina-b, MGC53005, ina-a, inexa, MGC82496, ina, XNIF, CTDSPL, MGC69277, INA, MGC142838, LOC555251, nef5, xina, nf-66, txbp-1

Clonality: Monoclonal (2-00E-03)

Host: Mouse

Reactivity: Mammalian

Application: Western Blotting (WB)

Catalog no.: ABIN306959

Additional Information

Alternative name: alpha-Internexin

Isotype: IgG1  (Matching secondary antibodies)

Clone: 2-00E-03

Description: Alpha-internexin is a Class IV intermediathee filament originally discovered as it copurifies with other neurofilament subunits (1). On SDSPAGE gels it runs with an apparent molecular weight of 64 to 66 kDa, with some species variability, although the real molecular weight is about 55kDa, as with the other neurofilament subunits the presence of highly negatively charged sequences results in reduction of SDS-PAGE mobility. a-internexin is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. It is expressed only in neurons and in large amounts early in neuronal development, but is down-regulated in many neurons as development procedes. Many classes of mature neurons contain a-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons a-internexin is the only neurofilament subunit expressed. Antibodies to a-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition the very early developmental expression of a-internexin means its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cell committed to the neuronal lineage. In addition recent studies show a marked up-regulation of a-internexin during neuronal regeneration (2). Synonyms: NF66.

Synonyms: NEF5, NF-66, TXBP-1, FLJ18662, FLJ57501, MGC12702, Nf66, Inexa, Intlaa, NF66, AV028420, MGC25352, Ina, cb126, sb:cb126, ina-A, xefiltin, ina-b, MGC53005, ina-a, inexa, MGC82496, ina, XNIF, CTDSPL, MGC69277, INA, MGC142838, LOC555251, nef5, xina, nf-66, txbp-1

Application Details

Application Notes: Antibody can be used for Western blotting (1:10,000), immunofluroescence and immunohistochemistry on frozen and paraffin-embedded formalin-fixed sections (1:5000). Positive Control: Human alpha-internexin.

Concentration: 1 mg/ml

Storage: Product should be stored at -20C. Aliquot to avoid freeze/thaw cycles

Restrictions: For Research Use only