Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F) (pSer5) antibody

Details for Product No. ABIN3071654, Supplier: Log in to see
Antigen
  • HRBP14.4
  • POLRF
  • RPABC2
  • RPB14.4
  • RPB6
  • 1810060D16Rik
  • polymerase (RNA) II (DNA directed) polypeptide F
  • POLR2F
  • Polr2f
Alternatives
anti-Yeast (Saccharomyces cerevisiae) Polymerase (RNA) II (DNA Directed) Polypeptide F antibody for Chromatin Immunoprecipitation
Epitope
pSer5
2
2
1
1
1
Reactivity
Human, Yeast (Saccharomyces cerevisiae)
24
11
8
2
1
1
1
1
1
1
Host
Mouse
10
10
4
Clonality
Monoclonal
Application
Chromatin Immunoprecipitation (ChIP), ELISA, Immunofluorescence (IF), Western Blotting (WB)
21
10
7
6
5
5
3
3
2
2
1
1
Options
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Specificity Recognises the phosphorylated (Serine 5) form of RNA polymerase II in Human and Saccharomyces cerevisiae.
Purification Protein A (affinity purified)
Alternative Name RNA Pol II (POLR2F Antibody Abstract)
Background RNA polymerase II catalyses the transcription of DNA into mRNA and other small nuclear RNAs in eukaryotes. It contains a CTD consisting of conserved heptapeptide repeats. Phosphorylation occurs at serine and threonine residues located in the CTD repeats to activate the RNA pol II. RNA pol II binds to several transcription factors in order to initiate transcription and therefore serves as an abundant antibody target for ChIP.
Pathways Regulatory RNA Pathways
Application Notes Chromatin Immunoprecipitation: 2 μg: 1 μg (antibody: chromatin)
Please note: Optimal antibody dilutions should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.95 mg/mL
Buffer Purified antibody in PBS + 0.1 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid multiple free/thaw cycles as this may denature the antibody
Storage -20 °C
Expiry Date 12 months
Supplier Images
Chromatin Immunoprecipitation (ChIP) image for anti-Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F) (pSer5) antibody (ABIN3071654) Chromatin immunoprecipitation (ChIP) assays were performed using the standard ChIP s...