TJP1 / ZO-1 (AA 334-634) antibody

Antigen: TJP1 / ZO-1 (AA 334-634)

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN307187

Additional Information

Swiss-Prot: Q07157

Immunogen: Human recombinant ZO-1 fusion protein encompassing amino acids 334-634.

Isotype: IgG1, kappa chain  (Matching secondary antibodies)

Description: Gene: TJP1 / ZO-1, Standard Gene Symbol: TJP1, Family: Occludin, Synonyms: TJP1, DKFZp686M05161, MGC133289, Tight junction protein zo-1, ZO-1

Specificity: Recognizes ZO-1a+ and ZO-1a- isoforms. Cross-reactivity with the related ZO-2 protein has not been observed. Species Cross-reactivity: Human and canine Predicted Reactivities: Dog (Canine).

Application Details

Application Notes: ELISA (0.1 - 1 µg/ml), IF (15 - 20 µg/ml), IHC-P (5 µg/ml), WB (1 - 2 µg/ml)

Concentration: 0.5 mg/ml

Purification: Protein A Column

Buffer: PBS, pH 7.4, 0.1% sodium azide.

Storage: Long term: -20°C, Short term: +4°C

Restrictions: For Research Use only

Images

Human Testis (formalin-fixed, paraffin-embedded) stained with TJP1 antibody ABIN307187 at 5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody ABIN481714, alkaline phosphatase-streptavidin and chromogen.

Human Kidney (formalin-fixed, paraffin-embedded) stained with TJP1 antibody ABIN307187 at 5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody ABIN481714, alkaline phosphatase-streptavidin and chromogen.