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Antibodies
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anti-Choline O-Acetyltransferase (CHAT) antibody
Choline O-Acetyltransferase (CHAT) antibody
| Antigen |
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| Synonyms |
CMS1A, CMS1A2, B230380D24Rik, CT23399, Cat, ChAT, cha, dChAT, l(3)91Cc, DmelCG12345, CG12345, CHAT, ChoAcTase, Ci-ChAT |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Human (45), Rat (Rattus) (36), Mouse (Murine) (35), Dog (Canine) (19), Pig (Porcine) (18), Guinea Pig (10), Chicken (4), Rabbit (4), Bird (Avian) (2), Cow (Bovine) (2), Horse (Equine) (2), Primates (2), Cat (Feline) (1), Monkey (1)
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Application
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 Alternatives Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
Western Blotting (WB) (40), Immunohistochemistry (IHC) (25), Immunofluorescence (IF) (20), Flow Cytometry (FACS) (18), Immunohistochemistry (Frozen Sections) (IHC (fro)) (16), ELISA (9), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (7), Enzyme Immunoassay (EIA) (5), Immunocytochemistry (ICC) (4), Immunoprecipitation (IP) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (EM) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Paraformaldehyde) (IHC (pfa)) (1)
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| Catalog no. |
ABIN308174 |
| Quantity |
100µl (Variants) |
| Price |
353.64 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Immunogen
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A 22 amino acid synthetic peptide from porcine ChAT, coupled to KLH. The peptide sequence is GLFSSYRLPGHTQDTLVAQKSS. Due to sequence similarity, the antibody is expected to react with pig, rat, mouse and human ChAT.Produced In:NZ white rabbit.
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Format
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Antiserum, Lyophilized
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Description
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Cholinergic systems are implicated in numerous neurologic functions. Alteration in some cholinergic neurons may account for the disturbances of Alzheimer disease. The protein encoded by this gene synthesizes the neurotransmitter acetylcholine. Alternative splice variants have been found that contain alternative 5' untranslated exons. Three of the four described splice variants encode identical 69 kDa proteins while one variant encodes both the 69 kDa and a larger 82 kDa protein. Choline acetylase catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. CATALYTIC ACTIVITY: Acetyl-CoA + choline = CoA + O-acetylcholine. Defects in CHAT are the cause of familial infantile myasthenia gravis 2 (FIMG2); also known as CMS-EA. FIMG2 patients have myasthenic symptoms since birth or early infancy, negative tests for anti-AChR antibodies, and abrupt episodic crises with increased weakness, bulbar paralysis, and apnea precipitated by undue exertion, fever, or excitement. Inheritance is autosomal recessive.
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Specificity
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Will stain cholinergic neurons in rat central and peripheral nervous systems. Not yet tested against human material but other rabbit and sheep antibodies against the same peptide have been shown to react against primate and human enzyme.
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Application Notes
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Immunohistochemistry, Western blot and 1 site ELISA (1:1000). Immunohistochemistry: Optimal at dilutions of 1:2000 to 1:4000
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Purity
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Whole Serum
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Storage
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Long term - 70ºC dry Short term - 20ºC solution or 4ºC with appropriate antibacterial agent. Avoid repeated freezing and thawing. Glycerol (1:1) can be added for additional stability. Reconstitute with addition of 100µl of sterile water.
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Research Area
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Alzheimer's Disease
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Restrictions
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For Research Use only
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Alternatives for antigen "Choline O-Acetyltransferase (CHAT)", type "Antibodies"
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Hosts
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Rabbit (42), Goat (15), Sheep (7), Mouse (6), Chicken (5)
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Reactivities
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Human (45), Rat (Rattus) (36), Mouse (Murine) (35), Dog (Canine) (19), Pig (Porcine) (18), Guinea Pig (10), Chicken (4), Rabbit (4), Bird (Avian) (2), Cow (Bovine) (2), Horse (Equine) (2), Primates (2), Cat (Feline) (1), Monkey (1)
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Applications
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Western Blotting (WB) (40), Immunohistochemistry (IHC) (25), Immunofluorescence (IF) (20), Flow Cytometry (FACS) (18), Immunohistochemistry (Frozen Sections) (IHC (fro)) (16), ELISA (9), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (7), Enzyme Immunoassay (EIA) (5), Immunocytochemistry (ICC) (4), Immunoprecipitation (IP) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (EM) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Paraformaldehyde) (IHC (pfa)) (1)
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Conjugates
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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C-Term (2), internal (2), N-Term (1)
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