Sequestosome 1 (SQSTM1) antibody

Antigen: Sequestosome 1 (SQSTM1)

Synonyms: p60, p62, A170, OSIL, PDB3, ZIP3, p62B, Osi, STAP, OSF-6, SQSTM1, MGC127197, MGC64432, sb:cb621, zgc:85784, DKFZp468L2213, sqstm1, MGC79491

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN308184

Additional Information

Alternative name: SQSTM1

Immunogen: Synthetic peptide as part of human Ubiquitin Interactive Motif (UIM) derived from p62 protein, also known as SQSTM1. (accession: AAH01874). Produced in: NZ white rabbit

Format: Lyophilized

Description: This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Multiple alternatively spliced variants, encoding the same protein, have been identified. Mutations in this gene result in sporadic and familial Paget disease of bone.

Specificity: Confirmed by immunohistochemical/western blot analysis of normal and diseased human brain.

Application Details

Application Notes: Western blot: The antibody recognizes 4 major bands of 130, 62, 45 and 22 kDa in human brain homogenate. The antibody may also recognize some proteins containing SQSTM1 domains from other species as 80, 62 and 47 kDa proteins are identified in rat sympathetic ganglia. Preincubation of the antibody with the immunizing peptide abolishes all reactivity in both human brain homogenate and rat sympathetic ganglia. Immunohistochemistry: Use at 1:500 to 1:2000 dilution. The end user should determine the optimal dilution. This antiserum works superbly in both cryosections and paraffin embedded tissues.

Purity: Whole Serum

Storage: Long term - 70ºC dry Short term - 20ºC solution or 4ºC with appropriate antibacterial agent. Avoid repeated freezing and thawing. Glycerol (1:1) can be added for additional stability. Reconstitute with addition of 50 µl of sterile water.

Restrictions: For Research Use only

Images

10 uM paraffin embedded sections were cut from Human post-mortem brain (cortex) and incubated with affinity purified rabbit anti-SQSTM1 antibody (1ug/ml, overnight at room temperature), biotinylated anti-rabbit secondary antibody and counterstained with Haematoxylin to identify nuclei (blue). (A): Alzheimer's disease: Neurofibrillary tangles (dark brown) are intensely stained by this antibody (20x objective)

Multiple system atrophy: Glial cytoplasmic inclusions are intensely stained by this antibody (dark brown) (20x objective)

Parkinson's Disease/Lewy Body dementia: Lewy bodies are intensely staind (dark brown). (20x objective)