Npy antibody
| Antigen |
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| Synonyms |
0710005A05Rik |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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Alternatives Immunohistochemistry (IHC), Radioimmunoassay (RIA)
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| Catalog no. |
ABIN308193 |
| Quantity |
100µl |
| Price |
337.60 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Immunogen
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Synthetic peptide of NPY coupled to Bovine Thyroglobulin. Produced in: Sheep
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Format
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Antiserum, Lyophilized
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Specificity
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The specificity of the NPY antiserum was determined by radioimmunoassay. Synthetic peptides, somatostatin-14, leu-enkephalin, met-enkephalin, oxytocin, Arg8-vasopressin, vasoactive intestinal peptide, angiotensin II demonstrated less than 0.005% cross- reactivity and substance P less than 0.02%. Peptide YY and bovine pancreatic polypeptide demonstrated approx. 36% and 0.05% cross- reactivity respectively. Human peptide YY share 65% homology amino acid sequence for human NPY. Synthetic NPY completely abolished radio labelled NPY binding to NPY antibody in RIA and antibody binding to tissue antigen.
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Application Notes
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Immunohistochemistry and Radioimmunoassay. Immunohistochemistry: Optimal at 1:5000 dilution in rabbit and guinea pig with avidin-bidin detection.
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Purity
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Whole Serum
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Storage
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Long term - 70ºC dry Short term - 20ºC solution or 4ºC with appropriate antibacterial agent. Avoid repeated freezing and thawing. Glycerol (1:1) can be added for additional stability. Reconstitute with addition of 100µl of sterile water.
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Restrictions
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For Research Use only
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Alternatives for antigen "Npy", type "Antibodies"
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Hosts
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Rabbit (16), Goat (2), Mouse (1)
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Reactivities
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Human (17), Rat (Rattus) (16), Cow (Bovine) (15), Horse (Equine) (15), Mouse (Murine) (15), Rabbit (15)
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Applications
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Flow Cytometry (FACS) (13), Immunofluorescence (IF) (13), Western Blotting (WB) (6), ELISA (4), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunohistochemistry (Frozen Sections) (IHC (fro)) (2), Immunoprecipitation (IP) (2), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (IHC) (1)
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Conjugates
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Alexa Flour 488 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1), RBITC (1)
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