HB9 antibody

Antigen: HB9

Clonality: Polyclonal

Host: Sheep

Reactivity: Human, Mouse (Murine)

Application: Immunohistochemistry (IHC), ELISA, Western Blotting (WB)

Catalog no.: ABIN308211

Additional Information

Immunogen: A 20 amino acid synthetic HB9 peptide (H-APADRLRAESPSPPRLLAAHC-NH2), conjugated to Blue Carrier Protein. Produced in: Sheep

Format: Lyophilized

Description: The homeobox gene Hb9/Hlxb9, is expressed selectively by motor neurons (MNs) in the developing vertebrate CNS. Hb9, which encodes a factor central to MN development regulates neuronal fate by restricting expression of Lim3 and Even-skipped (Eve), two homeodomain (HD) proteins required for development of distinct neuronal classes. In the vertebrate CNS, HB9/MNR2 transcription factors are specifically expressed in all somatic motoneurons and are necessary to distinguish motoneurons from interneurons. Hb9 is also a transcription factor expressed in lymphoid and pancreatic tissue. In humans, HB9 mutation causes an autosomal dominant form of sacral agenesis.

Specificity: Specific for HB9. Confirmed by Immunohistochemical analysis of Normal Term and First Trimester human Placenta as well as western blot analysis of mouse Neuroblastoma and Spinal Cord cells (NSC-34).

Application Details

Application Notes: ELISA (1:500 to 1:6500), Immunohistochemistry (1:1000), Western Blot (1:1000). Immunohistochemistry: Use at 1:500 to 1:2000 dilution. The end user should determine the optimal dilution.

Purity: Whole Serum

Storage: Long term - 70ºC dry Short term - 20ºC solution or 4ºC with appropriate antibacterial agent. Avoid repeated freezing and thawing. Glycerol (1:1) can be added for additional stability. Reconstitute with addition of 20 µl of sterile water. Centrifuge to remove any residues.

Research Area: Neurology

Restrictions: For Research Use only

Images

anti-HB9 antibody

anti-HB9 antibody (Image 2)