alpha-Methylacyl-CoA Racemase (AMACR) antibody

Antigen: alpha-Methylacyl-CoA Racemase (AMACR)

Synonyms: RACE, CBAS4, Macr1, Da1-8, Marc1, AMACR, DKFZp469O1232, amacr, MGC89832

Clonality: Polyclonal

Host: Various Hosts

Reactivity: Human

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN308237

Additional Information

Alternative name: PIN-4 (P63, Cytokeratin (HMW), p504S (AMACR))

Description: This reagent is a cocktail containing the three (3) antibodies described below. Monoclonal mouse antibody to human P63. Anti-P63 is a monoclonal mouse antibody, clone 4A4, produced by immunization with a recombinant protein derived from amino acid 1-205 of human p63. The isotype is IgG2a, kappa. This antibody recognizes a 63 kD protein, which is highly expressed in the basal or progenitor layers of many epithelial tissues. Monoclonal mouse antibody to human cytokeratin (HMW). The anti-cytokeratin (HMW) is a monoclonal mouse antibody, clone 34?E12, produced from immunization with solubilized keratin extract from human stratum corneum. The isotype is IgG1, kappa. This antibody recognizes keratin polypeptides of 68, 58, 56.5 and 50 kD in stratum corneum extracts. The antibody reacts with squamous, ductal and other complex epithelia. Rabbit anti-p504S (AMACR) antibody. Anti-p504S (AMACR) is a polyclonal rabbit antibody, produced by immunization with synthetic human AMACR peptide. The antibody comes from a purified immunoglobulin fraction of rabbit antiserum against AMACR. P504S (AMACR) encodes a protein involved in the beta-oxidation of branched chain fatty acids.

Specificity: The combined detection of p504S, p63 and high molecular weight cytokeratin markers has been shown to be useful for distinguishing benign conditions mimicking cancer from prostate carcinomas. In particular, these markers have been shown to be relevant in diagnosing the premalignant condition, prostatic intraepithelial neoplasia (PIN). High molecular weight cytokeratin and p63 are commonly used markers of basal epithelial cells. Benign prostate tissue contains basal cells, which are absent in prostate cancers. P504S has been recently described as a prostate cancer-specific gene. Expression of p504S (AMACR) protein is seen in prostatic adeno-carcinoma but not in benign prostatic tissue. Anti-p540S stains premalignant lesions of prostate: high-grade PIN and atypical adenomatous hyperplasia. The PIN-4 antibody combination may be particularly useful for confirming the diagnosis of prostate carcinoma in small foci of needle biopsies. The positive prostate cancer marker, p504S (AMACR), in conjunction with the negative basal cell markers (p63 and HMW cytokeratin) offers utility in diagnosing PIN in difficult cases where tissue may be limiting.

Application Details

Application Notes: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation time and conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. This cocktail should be detected with sequential application of UnoVueTM 1-Step Polymer Mouse HRP and UnoVueTM 1-Step Rabbit AP Polymer rather than broad spectrum polymer reagents. This antibody product has been optimized for dual staining immunohistochemistry on automated stainers (e.g. Leica, DAKO). Contact DBS Technical Support for detailed double-staining protocols. Positive Control: Prostatic intraepithelial neoplasia (PIN). Cellular Localization: P63: Nuclear, Cytokeratin (HMW): Cytoplasmic, P504S (AMACR): Cytoplasmic. Intended Use: This product is intended for qualitative immunohistochemistry with normal and neoplastic formalin-fixed, paraffin-embedded tissue sections, to be viewed by light microscopy. Clinical interpretation of staining results should be accompanied by histological studies with proper controls. Patients

Concentration: 100ug/ml approximately

Storage: Store the antibody at 2-8

Research Area: Cancer

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union