MYOGENIN (MYOG) antibody

Antigen: MYOGENIN (MYOG)

Synonyms: MYF4, bHLHc3, MYOGENIN

Clonality: Monoclonal (F5D)

Host: Mouse

Reactivity: Mouse (Murine), Rat (Rattus), Cat (Feline)

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN308238

Additional Information

Alternative name: Myogenin

Isotype: IgG1  (Matching secondary antibodies)

Clone: F5D

Description: This reagent is a cocktail containing the three (3) antibodies described below. Monoclonal mouse antibody to human P63. Anti-P63 is a monoclonal mouse antibody, clone 4A4, produced by immunization with a recombinant protein derived from amino acid 1-205 of human p63. The isotype is IgG2a, kappa. This antibody recognizes a 63 kD protein, which is highly expressed in the basal or progenitor layers of many epithelial tissues. Monoclonal mouse antibody to human cytokeratin (HMW). The anti-cytokeratin (HMW) is a monoclonal mouse antibody, clone 34?E12, produced from immunization with solubilized keratin extract from human stratum corneum. The isotype is IgG1, kappa. This antibody recognizes keratin polypeptides of 68, 58, 56.5 and 50 kD in stratum corneum extracts. The antibody reacts with squamous, ductal and other complex epithelia. Rabbit anti-p504S (AMACR) antibody. Anti-p504S (AMACR) is a polyclonal rabbit antibody, produced by immunization with synthetic human AMACR peptide. The antibody comes from a purified immunoglobulin fraction of rabbit antiserum against AMACR. P504S (AMACR) encodes a protein involved in the beta-oxidation of branched chain fatty acids.

Specificity: This antibody is specific to 34 kD protein known as Myogenin. Myogenin is a member of family of myogenic regulatory genes, which includes MyoD, myf5 and MRF4. These genes encode a set of transcription factors, which are essential for muscle development. Expression of myogenin is restricted to cells of skeletal muscle origin. This antibody cross reacts with mouse, rat and cat.

Application Details

Application Notes: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate, pH 6.0 or 1 mM EDTA, pH 8 buffer prior to immunostaining. Positive Control: Rhabdomyosarcoma. Cellular Localization: Nuclear

Concentration: 1 to 4 ug/ml

Storage: Store the antibody at 2-8

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Wright, Sassoon, Lin: "Myogenin, a factor regulating myogenesis, has a domain homologous to MyoD." in: Cell, Vol. 56, Issue 4, pp. 607-17, 1989 (PubMed).

Auradue, Pinset, Chafey et al.: "Myf5, MyoD, myogenin and MRF4 myogenic derivatives of the embryonic mesenchymal cell line C3H10T1/2 exhibit the same adult muscle phenotype." in: Differentiation; research in biological diversity, Vol. 55, Issue 3, pp. 185-92, 1994 (PubMed).

Wright, Dac-Korytko, Farmer: "Monoclonal antimyogenin antibodies define epitopes outside the bHLH domain where binding interferes with protein-protein and protein-DNA interactions." in: Developmental genetics, Vol. 19, Issue 2, pp. 131-8, 1997 (PubMed).