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Fat Mass and Obesity-Associated (FTO) (Internal Region) antibody

Details for Product No. ABIN308414, Supplier: Login to see
Antigen
  • AW743446
  • mKIAA1752
  • RGD1305121
Epitope
Internal Region
40
12
6
5
4
2
1
1
1
Reactivity
Human
108
58
37
10
7
6
6
5
4
3
1
1
Host
Goat
83
24
15
5
1
Clonality
Polyclonal
Conjugate
Un-conjugated
4
4
4
3
3
3
1
1
1
1
1
1
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
107
69
38
14
14
11
9
8
3
1
Supplier
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Immunogen Peptide with sequence QQKPDCRPYWEKDD, from the Internal region of the protein sequence according to NP_036066.2.
Blocking Peptide Blocking peptide for this product available: ABIN370403
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine), Pig (Porcine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name FTO (FTO Antibody Abstract)
Background Synonyms: Fto, fat mass and obesity associated, AW743446, mKIAA1752, similar to FTO
Gene ID 79068, 26383, 291905
NCBI Accession NP_036066
Pathways
Application Notes ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx. 60 kDa band observed in Human Pancreas lysates (calculated MW of 58.0 kDa according to NP_036066.2). Recommended concentration: 0.03-0.1 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-Fat Mass and Obesity-Associated (FTO) (Internal Region) antibody (ABIN308414) ABIN308414 (0.03µg/ml) staining of Human Pancreas lysate (35µg protein in RIPA buffer...
Background publications Gerken, Girard, Tung et al.: "The obesity-associated FTO gene encodes a 2-oxoglutarate-dependent nucleic acid demethylase. ..." in: Science (New York, N.Y.), Vol. 318, Issue 5855, pp. 1469-72, 2007 (PubMed).