Fat Mass and Obesity-Associated (FTO) (Internal Region) antibody

Details for Product No. ABIN308414
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Antigen
Synonyms AW743446, mKIAA1752, RGD1305121
Epitope
Internal Region
(26), (10), (7), (4), (2), (2), (1), (1)
Reactivity
Human
(62), (40), (26), (15), (14), (12), (9), (3), (3)
Host
Goat
(55), (11), (4), (2), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(63), (37), (21), (9), (8), (7), (5), (4), (1)
Pubmed 1 reference available
Quantity 100 µg
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Catalog No. ABIN308414
374.27 $
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Immunogen Peptide with sequence QQKPDCRPYWEKDD, from the Internal region of the protein sequence according to NP_036066.2.
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine), Pig (Porcine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name FTO
Background Synonyms: Fto, fat mass and obesity associated, AW743446, mKIAA1752, similar to FTO
Gene ID 79068, 26383, 291905
NCBI Accession NP_036066
Application Notes ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx. 60 kDa band observed in Human Pancreas lysates (calculated MW of 58.0 kDa according to NP_036066.2). Recommended concentration: 0.03-0.1 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Fat Mass and Obesity-Associated (FTO) (Internal Region) antibody ABIN308414 (0.03µg/ml) staining of Human Pancreas lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Gerken, Girard, Tung et al.: "The obesity-associated FTO gene encodes a 2-oxoglutarate-dependent nucleic acid demethylase." in: Science (New York, N.Y.), Vol. 318, Issue 5855, pp. 1469-72, 2007 (PubMed).

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