IQ Motif Containing GTPase Activating Protein 1 (IQGAP1) (Internal Region) antibody

Details for Product No. ABIN308431
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Antigen
Synonyms HUMORFA01, SAR1, p195, AA682088, D7Ertd237e, D7Ertd257e, mKIAA0051
Epitope
Internal Region
(15), (7), (7), (4), (2), (2), (1), (1), (1), (1)
Reactivity
Mouse (Murine)
(57), (29), (18), (1)
Host
Goat
(39), (14), (6)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(48), (32), (10), (10), (10), (9), (8), (7), (6), (1)
Pubmed 1 reference available
Quantity 100 μg
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Catalog No. ABIN308431
374.27 $
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Immunogen Peptide with sequence C-HSRLVESKVELNA, from the internal region of the protein sequence according to NP_064535.1.
Cross-Reactivity Human, Rat (Rattus)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name SAR1
Background Synonyms: SAR1A, SAR1 gene homolog A (S. cerevisiae), SAR1, SARA1, Sara, masra2, OTTHUMP00000060603, SAR1a gene homolog 1
Gene ID 56681, 20224, 361842
NCBI Accession NP_064535
Research Area Signaling
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx. 23 kDa band observed in lysates of NIH3T3 (calculated MW of 22.4 kDa according to Human NP_064535.1 and to Mouse NP_033146.1). Recommended concentration: 0.1-0.3 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-IQ Motif Containing GTPase Activating Protein 1 (IQGAP1) (Internal Region) antibody ABIN308431 (0.1µg/ml) staining of NIH3T3 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Bi, Mancias, Goldberg: "Insights into COPII coat nucleation from the structure of Sec23.Sar1 complexed with the active fragment of Sec31." in: Developmental cell, Vol. 13, Issue 5, pp. 635-45, 2007 (PubMed).

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