Collagen, Type IV, alpha 3 (Goodpasture Antigen) Binding Protein (COL4A3BP) (AA 396-411) antibody

Antigen: Collagen, Type IV, alpha 3 (Goodpasture Antigen) Binding Protein (COL4A3BP)

Synonyms: COL4A3BP, col4a3bp, zgc:92147, wu:fc37f11, CERT, GPBP, AU016711, 2810404O15Rik, 9230101K08Rik, CERTL, STARD11, FLJ20597, cetp, MGC80009

Epitope: AA 396-411

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN308432

Additional Information

Alternative name: COL4A3BP (aa396-411)

Gene ID: 10087

Immunogen: C-KREDSWQKRLDKETEK

Format: Affinity-purified

Description: All names and symbols: COL4A3BP, collagen, type IV, alpha 3 (Goodpasture antigen) binding protein, CERT, CERTL, FLJ20597, GPBP, STARD11, Goodpasture antigen-binding protein, START domain containing 11, StAR-related lipid transfer (START) domain containing 11, alpha 3 type IV collagen binding protein, ceramide transporter, lipid-transfer protein CERTL. Official Symbol: COL4A3BP. RefSeq number(s): NP_005704.1, NP_112729.1

Specificity: This antibody is expected to recognize both reported isoforms (NP_005704.1, NP_112729.1). Human, Dog, Cow

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:2,000. Western Blot: Approx. 70kDa band observed in Human Heart lysates (calculated MW of 70.8kDa according to NP_005704.1). Recommended concentration: 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN308432 (1µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Kudo, Kumagai, Tomishige et al.: "Structural basis for specific lipid recognition by CERT responsible for nonvesicular trafficking of ceramide." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 2, pp. 488-93, 2008 (PubMed).