PCSK6 antibody (Internal Region)

Catalog No. ABIN308447

Product Details anti-PCSK6 Antibody

Target: PCSK6 (Proprotein Convertase Subtilisin/kexin Type 6 (PCSK6))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PCSK6 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Purpose: PACE4

Sequence: PDCEPGTYFD SE

Specificity: This antibody is expected to recognize reported isoforms a, b, g, h (NP_002561.1, NP_612192.1, NP_612193.1, NP_612194.1 resp.).

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence PDCEPGTYFDSE, from the internal region of the protein sequence according to NP_002561.1, NP_612192.1, NP_612195.1, NP_612197.1, NP_612196.1, NP_612198.2, NP_612193.1, NP_612194.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Testis and Skeletal Muscle. Recommended concentration: 3.75 μg/mL.

Western Blot: Preliminary experiments gave an approx. 70 kDa band in Human, Mouse and Rat Liver lysates after 1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-PCSK6 antibody (ABIN308447)

Ha, Jin, Jorgensen, Zogg, Wei, Singh, Park, Kurahashi, Kim, Baek, Poudrier, Lee, Sanders, Ro: "Transcriptome profiling of subepithelial PDGFRα cells in colonic mucosa reveals several cell-selective markers." in: PloS one, Vol. 17, Issue 5, pp. e0261743, (2022) (PubMed).

Target Details for PCSK6

Target: PCSK6 (Proprotein Convertase Subtilisin/kexin Type 6 (PCSK6))

Alternative Name: PCSK6 (PCSK6 Products)

Synonyms: PACE4 antibody, PCSK6 antibody, SPC4 antibody, C86343 antibody, Pace4 antibody, Spc4 antibody, PACE4AIIa antibody, Xpace4 antibody, spc4 antibody, proprotein convertase subtilisin/kexin type 6 antibody, peptidase S8 antibody, proprotein convertase subtilisin/kexin type 6 L homeolog antibody, PCSK6 antibody, EAMY_RS27925 antibody, pcsk6 antibody, Pcsk6 antibody, pcsk6.L antibody

Background: PCSK6, proprotein convertase subtilisin/kexin type 6, PACE4, SPC4, paired basic amino acid cleaving system 4, subtilisin-like proprotein convertase 4, subtilisin-like protease, subtilisin/kexin-like protease PACE4

Gene ID: 5046, 18553, 25507

NCBI Accession: NP_002561, NP_612192, NP_612195, NP_612197, NP_612196, NP_612198, NP_612193, NP_612194

Pathways: Neurotrophin Signaling Pathway, SARS-CoV-2 Protein Interactome