TFB2M antibody (N-Term)

Catalog No. ABIN309737

Product Details anti-TFB2M Antibody

Target: TFB2M (Transcription Factor B2, Mitochondrial (TFB2M))

Binding Specificity: N-Term

Reactivity: Human, Rat, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TFB2M antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: ECNPGPGILT QALLEAGAKV VALESDKTFI PHLESLGKNL DGKLRVIHCD

Predicted Reactivity: Horse: 100%, Human: 100%, Rabbit: 83%, Rat: 85%

Characteristics: This is a rabbit polyclonal antibody against TFB2M. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human TFB2M

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 396 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for TFB2M

Target: TFB2M (Transcription Factor B2, Mitochondrial (TFB2M))

Alternative Name: TFB2M (TFB2M Products)

Synonyms: CG3910 antibody, Dmel\\CG3910 antibody, TFB2 antibody, d-TF2BM antibody, d-mtTFB2 antibody, TFB2M antibody, sb:cb857 antibody, hkp1 antibody, Hkp1 antibody, mtTFB2 antibody, mitochondrial transcription factor B2 antibody, transcription factor B2, mitochondrial antibody, mtTFB2 antibody, TFB2M antibody, tfb2m antibody, Tfb2m antibody

Background: TFB2M is a S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA at the conserved stem loop. It is also required for basal transcription of mitochondrial DNA, probably via its interaction with POLRMT and TFAM. It stimulates transcription independently of the methyltransferase activity. Compared to TFB1M, it activates transcription of mitochondrial DNA more efficiently, while it has less methyltransferase activity.
Alias Symbols: FLJ22661, FLJ23182, Hkp1, mtTFB2
Protein Interaction Partner: UBC, FBXO6, C1QBP, CUL3, TFB2M, POLRMT, TFAM, PNP,
Protein Size: 396

Molecular Weight: 45 kDa

Gene ID: 64216

NCBI Accession: NM_022366, NP_071761

UniProt: Q9H5Q4