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Regulatory Factor X 4 (RFX4) (N-Term) antibody

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Antigen
Synonyms NYD-SP10, 4933412G19Rik, NYD-sp10, RGD1562092
Epitope
N-Term
(7), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus), Zebrafish (Danio rerio)
(31), (20), (19), (4), (2), (1)
Host
Rabbit
(31), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(22), (11), (4), (3)
Pubmed 1 reference available
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Quantity 50 μg
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Immunogen Synthetic peptide directed towards the N terminal of human RFX4
Blocking Peptide Blocking peptide for this product available: ABIN982892
Sequence QQFPQLTTRR LGTRGQSKYH YYGIAVKESS QYYDVMYSKK GAAWVSETGK
Predicted Reactivity Bovine : 100 %, Dog : 100 %, Guinea pig : 100 %, Horse : 100 %, Human : 100 %, Mouse : 100 %, Pig : 100 %, Rabbit : 100 %, Rat : 100 %, Zebrafish : 100 %, Chicken : 90 %
Characteristics This is a rabbit polyclonal antibody against RFX4. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name RFX4 (RFX4 Antibody Abstract)
Background RFX4 is a transcription factors that contain a highly-conserved winged helix DNA binding domain. RFX4 is structurally related to regulatory factors X1, X2, X3, and X5. It has been shown to interact with itself as well as with regulatory factors X2 and X3, but it does not interact with regulatory factor X1. RFX4 may be a transcriptional repressor rather than a transcriptional activator.This gene is a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory factors X1, X2, X3, and X5. It has been shown to interact with itself as well as with regulatory factors X2 and X3, but it does not interact with regulatory factor X1. This protein may be a transcriptional repressor rather than a transcriptional activator. Three transcript variants encoding different isoforms have been described for this gene.This gene is a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory factors X1, X2, X3, and X5. It has been shown to interact with itself as well as with regulatory factors X2 and X3, but it does not interact with regulatory factor X1. This protein may be a transcriptional repressor rather than a transcriptional activator. Three transcript variants encoding different isoforms have been described for this gene.
Molecular Weight 64 kDa
Gene ID 5992
NCBI Accession NM_002920, NP_002911
UniProt Q33E94
Research Area Chromatin and Nuclear Signaling, Transcription Factors
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 563 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
Western Blotting (WB) image for anti-Regulatory Factor X 4 (RFX4) (N-Term) antibody (ABIN309965) anti-Regulatory Factor X 4 (RFX4) (N-Term) antibody
Background publications Zhang, Harry, Blackshear et al.: "G-protein pathway suppressor 2 (GPS2) interacts with the regulatory factor X4 variant 3 (RFX4_v3) and functions as a transcriptional co-activator." in: The Journal of biological chemistry, Vol. 283, Issue 13, pp. 8580-90, 2008 (PubMed).

Catalog No. ABIN309965
$289.00
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