Ribosomal Protein, Large, P0 (RPLP0) (N-Term) antibody

Details for Product No. ABIN310003
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Antigen
Synonyms
AP3, Ap3, Ape, CG7490, Dmel\\CG7490, LP0, P0, P35, PO, RLA0, RPL P0, RpP0, RpPO, Rplp0, l(3)0154, l(3)01544, p0, RPLP0, arp, fa56b12, fb04a12, wu:fa56b12, wu:fb15a08, wu:fk48c12, arbp, l10e, lp0, prlp ... show more
AP3, Ap3, Ape, CG7490, Dmel\\CG7490, LP0, P0, P35, PO, RLA0, RPL P0, RpP0, RpPO, Rplp0, l(3)0154, l(3)01544, p0, RPLP0, arp, fa56b12, fb04a12, wu:fa56b12, wu:fb15a08, wu:fk48c12, arbp, l10e, lp0, prlp0, rpp0, L10E, PRLP0, RPP0, 36B4, Arbp, ARBP show less
Epitope
N-Term
(8), (8), (2), (1), (1), (1), (1)
Reactivity
Mouse (Murine), Rat (Rattus), Zebrafish (Danio rerio), Xenopus laevis, Dog (Canine), Human, Chicken
(32), (17), (17), (13), (13), (13), (13), (1), (1)
Host
Rabbit
(31), (3)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(25), (12), (10), (4), (3), (3), (1), (1)
Pubmed 1 reference available
Quantity 50 µg
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Catalog No. ABIN310003
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Immunogen Synthetic peptide directed towards the N terminal of human RPLP0
Sequence TEIRDMLLAN KVPAAARAGA IAPCEVTVPA QNTGLGPEKT SFFQALGITT
Predicted Reactivity African clawed frog : 100 %, Bovine : 100 %, Chicken : 100 %, Dog : 100 %, Human : 100 %, Mouse : 100 %, Zebrafish : 100 %, Pig : 90 %
Characteristics This is a rabbit polyclonal antibody against RPLP0. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name RPLP0
Background Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. The ribosomal protein is a component of the 60S subunit. The protein, which is the functional equivalent of the E. coli L10 ribosomal protein, belongs to the L10P family of ribosomal proteins. It is a neutral phosphoprotein with a C-terminal end that is nearly identical to the C-terminal ends of the acidic ribosomal phosphoproteins P1 and P2. The P0 protein can interact with P1 and P2 to form a pentameric complex consisting of P1 and P2 dimers, and a P0 monomer. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein, which is the functional equivalent of the E. coli L10 ribosomal protein, belongs to the L10P family of ribosomal proteins. It is a neutral phosphoprotein with a C-terminal end that is nearly identical to the C-terminal ends of the acidic ribosomal phosphoproteins P1 and P2. The P0 protein can interact with P1 and P2 to form a pentameric complex consisting of P1 and P2 dimers, and a P0 monomer. The protein is located in the cytoplasm. Transcript variants derived from alternative splicing exist, they encode the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein, which is the functional equivalent of the E. coli L10 ribosomal protein, belongs to the L10P family of ribosomal proteins. It is a neutral phosphoprotein with a C-terminal end that is nearly identical to the C-terminal ends of the acidic ribosomal phosphoproteins P1 and P2. The P0 protein can interact with P1 and P2 to form a pentameric complex consisting of P1 and P2 dimers, and a P0 monomer. The protein is located in the cytoplasm. Transcript variants derived from alternative splicing exist, they encode the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
Molecular Weight 34 kDa
Gene ID 6175
NCBI Accession NP_000993, NM_001002
UniProt P05388
Research Area Chromatin and Nuclear Signaling, DNA/RNA
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 317 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Ribosomal Protein, Large, P0 (RPLP0) (N-Term) antibody WB Suggested Anti-RPLP0 Antibody Titration: 0.2-1 ug/ml
Positive Control: HepG2 cell lysate
anti-Ribosomal Protein, Large, P0 (RPLP0) (N-Term) antibody (2) Rabbit Anti-RPLP0 Antibody ,Paraffin Embedded Tissue: Human Bronchial Epithelial Tissue
Primary Antibody Concentration: 1:100
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:000
Magnification: 00X
Exposure Time: 0.5 - 2.0 sec
Background publications Rinne, Clements, Lamme et al.: "A novel translation re-initiation mechanism for the p63 gene revealed by amino-terminal truncating mutations in Rapp-Hodgkin/Hay-Wells-like syndromes." in: Human molecular genetics, Vol. 17, Issue 13, pp. 1968-77, 2008 (PubMed).

Validation Images
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