Aminolevulinate Dehydratase (ALAD) (Middle Region) antibody

Details for Product No. ABIN310192
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms DDBDRAFT_0190269, DDBDRAFT_0231415, DDB_0190269, DDB_0231415, ALAD, ncf, ALADH, PBGS, ALADR, aminolevulinatedelta-dehydratase, zgc:110219, Lv
Epitope
Middle Region
(13), (4), (3), (2), (2), (1), (1), (1)
Reactivity
Mouse (Murine), Rat (Rattus), Cow (Bovine), Human
(37), (15), (14), (13), (13), (1), (1)
Host
Rabbit
(35), (3)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(29), (9), (5), (4), (4), (4), (3), (1)
Pubmed 1 reference available
Quantity 50 μg
Options
Shipping to United States (Change)
Availability Will be delivered in 2 to 3 Business Days
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN310192
289.00 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Synthetic peptide directed towards the middle region of human ALAD
Sequence SVMSYSAKFA SCFYGPFRDA AKSSPAFGDR RCYQLPPGAR GLALRAVDRD
Predicted Reactivity Human : 100 %, Bovine : 90 %, Dog : 90 %, Guinea pig : 90 %, Mouse : 90 %, Rabbit : 90 %, African clawed frog : 80 %, Chicken : 80 %, Zebrafish : 80 %
Characteristics This is a rabbit polyclonal antibody against ALAD. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name ALAD
Background The ALAD enzyme is composed of 8 identical subunits and catalyzes the condensation of 2 molecules of delta-aminolevulinate to form porphobilinogen (a precursor of heme, cytochromes and other hemoproteins). ALAD catalyzes the second step in the porphyrin and heme biosynthetic pathway, zinc is essential for enzymatic activity. ALAD enzymatic activity is inhibited by lead and a defect in the ALAD structural gene can cause increased sensitivity to lead poisoning and acute hepatic porphyria.The ALAD enzyme is composed of 8 identical subunits and catalyzes the condensation of 2 molecules of delta-aminolevulinate to form porphobilinogen (a precursor of heme, cytochromes and other hemoproteins). ALAD catalyzes the second step in the porphyrin and heme biosynthetic pathway, zinc is essential for enzymatic activity. ALAD enzymatic activity is inhibited by lead and a defect in the ALAD structural gene can cause increased sensitivity to lead poisoning and acute hepatic porphyria. Alternatively spliced transcript variants encoding different isoforms have been identified.
Molecular Weight 39 kDa
Gene ID 210
NCBI Accession NM_001003945, NP_001003945
UniProt Q6ZMU0
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 359 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Aminolevulinate Dehydratase (ALAD) (Middle Region) antibody WB Suggested Anti-ALAD Antibody Titration: 0.2-1 ug/ml
Positive Control: Jurkat cell lysate
anti-Aminolevulinate Dehydratase (ALAD) (Middle Region) antibody (2) WB Suggested Anti-ALAD
Antibody Titration: 0.2-1 µg/mL
Positive Control: Jurkat cell lysate
ALAD is supported by BioGPS gene expression data to be expressed in Jurkat
Background publications Tang, Breinig, Stith et al.: "Single amino acid mutations alter the distribution of human porphobilinogen synthase quaternary structure isoforms (morpheeins)." in: The Journal of biological chemistry, Vol. 281, Issue 10, pp. 6682-90, 2006 (PubMed).

Validation Images
Did you look for something else?
back to top