anti-ALP antibody (Alkaline Phosphatase, Placental) (C-Term)

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Antigen
Epitope
C-Term
(29), (16), (13), (13), (6), (3), (3), (3), (2), (1), (1), (1), (1), (1)
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus), Xenopus laevis
(156), (11), (9), (1), (1), (1)
Host
Rabbit
(77), (77), (4)
Clonality
Polyclonal
Conjugate
This ALP antibody is un-conjugated
(9), (5), (5), (5), (5), (5), (2)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(105), (80), (65), (38), (20), (9), (8), (8), (6), (5), (1), (1), (1), (1)
Pubmed 2 references available
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Quantity 100 μg
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Immunogen Synthetic peptide directed towards the C terminal of human ALPP
Sequence TVLLYGNGPG YVLKDGARPD VTESESGSPE YRQQSAVPLD EETHAGEDVA
Predicted Reactivity Human :100 %,   African clawed frog :80 %,   Dog :80 %,   Western clawed frog :80 %,   Green puffer :80 %,   Yellowfever mosquito :80 %,   Shewanella baltica BA175 :80 %,   Shewanella baltica OS183 :80 %,   Shewanella baltica OS678 :80 %,   Shewanella benthica KT99 :80 %,   Pseudoalteromonas tunicata D2 :80 %,   Alteromonadales bacterium TW-7 :80 %,   Sea squirt :70 %,   Chicken :70 %,   Zebrafish :70 %,   Mouse :70 %,   Atlantic cod :70 %,   Rat :70 %,   Silk moth :70 %,   Wild silk moth :70 %,   Bovine :70 %,   Florida lancelet :70 %,   Savannah tsetse fly :70 %,   Congregibacter litoralis KT71 :70 %,   Black-legged tick :70 %,   Red flour beetle :70 %,   Gamma proteobacterium HTCC2207 :70 %,   Fruit fly :70 %,   Encephalitis mosquito :70 %,  
Characteristics This is a rabbit polyclonal antibody against ALPP. It was validated on Western Blot and immunohistochemistry.
Purification Protein A purified
Alternative Name ALPP (ALP Antibody Abstract)
Background There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. ALPP is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form.There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3' untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized.
Molecular Weight 59 kDa
Gene ID 250
NCBI Accession NP_001623, NM_001632
UniProt P05187
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 535 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 100 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
Western Blotting (WB) image for anti-ALP antibody (Alkaline Phosphatase, Placental) (C-Term) (ABIN310261) WB Suggested Anti-ALPP Antibody Titration: 1.25ug/ml
Positive Control: <...
Immunohistochemistry (IHC) image for anti-ALP antibody (Alkaline Phosphatase, Placental) (C-Term) (ABIN310261) anti-Alkaline Phosphatase, Placental (ALP) (C-Term) antibody (Image 2)
Product cited in: Lazrek, Goffard, Schanen et al.: "Detection of hepatitis C virus antibodies and RNA among medicolegal autopsy cases in Northern France." in: Diagnostic microbiology and infectious disease, Vol. 55, Issue 1, pp. 55-8, 2006 (PubMed).

Background publications Kimura, Wakamatsu, Suzuki et al.: "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. ..." in: Genome research, Vol. 16, Issue 1, pp. 55-65, 2006 (PubMed).

Catalog No. ABIN310261
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