Ring Finger Protein 40, E3 Ubiquitin Protein Ligase (RNF40) (C-Term) antibody

Details for Product No. ABIN310439, Supplier: Login to see New
New
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Epitope
C-Term
(19), (15), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(53), (12), (12), (4), (2), (1)
Host
Rabbit
(50), (3)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (2), (2), (2), (1)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(48), (26), (12), (5), (4), (3), (1), (1), (1), (1), (1)
Pubmed 1 reference available
Supplier Login to see New
Catalog number from supplier Login to see New
Quantity 100 μg
Options
Shipping to United States ( )
Availability
Immunogen Synthetic peptide directed towards the C terminal of human RNF40
Sequence KLLREEKDEL GEQVLGLKSQ VDAQLLTVQK LEEKERALQG SLGGVEKELT
Predicted Reactivity Bovine : 100 %, Dog : 100 %, Horse : 100 %, Human : 100 %, Rabbit : 100 %
Characteristics This is a rabbit polyclonal antibody against RNF40. It was validated on Western Blot and immunohistochemistry.
Purification Protein A purified
Alternative Name RNF40 (RNF40 Antibody Abstract)
Background RNF40 contains a RING finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein was reported to interact with the tumor suppressor protein RB1. Studies of the rat counterpart suggested that this protein may function as an E3 ubiquitin-protein ligase, and facilitate the ubiquitination and degradation of syntaxin 1, which is an essential component of the neurotransmitter release machinery.The protein encoded by this gene contains a RING finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein was reported to interact with the tumor suppressor protein RB1. Studies of the rat counterpart suggested that this protein may function as an E3 ubiquitin-protein ligase, and facilitate the ubiquitination and degradation of syntaxin 1, which is an essential component of the neurotransmitter release machinery.
Molecular Weight 114 kDa
Gene ID 9810
NCBI Accession NM_014771, NP_055586
UniProt O75150
Research Area Proteolysis / Ubiquitin, Ion Channels
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 1001 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 100 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
Western Blotting (WB) image for anti-Ring Finger Protein 40, E3 Ubiquitin Protein Ligase (RNF40) (C-Term) antibody (ABIN310439) WB Suggested Anti-RNF40 Antibody Titration: 1.25ug/ml
Positive Control: ...
Immunohistochemistry (IHC) image for anti-Ring Finger Protein 40, E3 Ubiquitin Protein Ligase (RNF40) (C-Term) antibody (ABIN310439) Human Muscle
Immunohistochemistry (IHC) image for anti-Ring Finger Protein 40, E3 Ubiquitin Protein Ligase (RNF40) (C-Term) antibody (ABIN310439) Sample Type: Adult mouse cerebellum (l0x) section

Primary Antibody Dilutio...
Background publications Zhu, Zheng, Pham et al.: "Monoubiquitination of human histone H2B: the factors involved and their roles in HOX gene regulation." in: Molecular cell, Vol. 20, Issue 4, pp. 601-11, 2005 (PubMed).