Sonic Hedgehog (SHH) (N-Term) antibody

Details for Product No. ABIN310574
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Antigen
Synonyms shh, SHH, 9530036O11Rik, Dsh, Hhg1, Hx, Hxl3, M100081, HHG1, HLP3, HPE3, MCOPCB5, SMMCI, TPT, TPTPS, Xhh, hedgehog, xshh, fc83d08, syu, vhh-1, vhh1, wu:fc83d08
Epitope
N-Term
(18), (5), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity
Mouse (Murine), Dog (Canine), Rat (Rattus), Xenopus laevis, Human, Zebrafish (Danio rerio), Chicken
(69), (39), (26), (12), (12), (3), (1), (1)
Host
Rabbit
(48), (29), (6), (3), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(5), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(64), (33), (27), (15), (14), (13), (10), (10), (8), (5), (2), (2), (1), (1), (1)
Pubmed 1 reference available
Quantity 50 μg
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Catalog No. ABIN310574
289.00 $
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Immunogen Synthetic peptide directed towards the N terminal of human SHH
Sequence RCLLLVLVSS LLVCSGLACG PGRGFGKRRH PKKLTPLAYK QFIPNVAEKT
Predicted Reactivity African clawed frog : 100 %, Chicken : 100 %, Dog : 100 %, Guinea pig : 100 %, Horse : 100 %, Human : 100 %, Mouse : 100 %, Pig : 100 %, Rat : 100 %, Zebrafish : 100 %, Bovine : 90 %
Characteristics This is a rabbit polyclonal antibody against SHH. It was validated on Western Blot and immunohistochemistry.
Purification Affinity Purified
Alternative Name SHH
Background SHH is a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE). It is also thought that mutations in its gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities.This gene, which is expressed only during embryogenesis, encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved, the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. In addition, it is thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities.
Molecular Weight 28 kDa
Gene ID 6469
NCBI Accession NP_000184, NM_000193
UniProt Q15465
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 462 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Note: This antibody may have up to 0.9% sodium azide in the buffer upon resuspension of the lyophilized pellet.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Sonic Hedgehog (SHH) (N-Term) antibody WB Suggested Anti-SHH Antibody Titration: 0.2-1 ug/ml
Positive Control: HepG2 cell lysate
anti-Sonic Hedgehog (SHH) (N-Term) antibody (2) Sample Type: Chicken EmbryosDilution: 1:1000
Background publications Coon, Roberts, Loscertales et al.: "Differential epithelial expression of SHH and FOXF1 in usual and nonspecific interstitial pneumonia." in: Experimental and molecular pathology, Vol. 80, Issue 2, pp. 119-23, 2006 (PubMed).

Validation Images
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