Replication Factor C (Activator 1) 3, 38kDa (RFC3) (N-Term) antibody

Details for Product No. ABIN310766
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Antigen
Synonyms CG5313, DRFC, DmRFC3, Dmel\\CG5313, Rfc3, 21.m02902, 2810416I22Rik, 38kDa, AU022547, Recc3, cb275, RFC38
Epitope
N-Term
(5), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Dog (Canine), Mouse (Murine), Rat (Rattus), Cow (Bovine), Xenopus laevis, Human, Chicken
(36), (9), (9), (1), (1), (1), (1), (1)
Host
Rabbit
(31), (7)
Clonality
Polyclonal
Application
Western Blotting (WB)
(36), (13), (6), (5), (4), (4), (3), (2), (1), (1)
Pubmed 1 reference available
Quantity 50 µg
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Catalog No. ABIN310766
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Immunogen Synthetic peptide directed towards the N terminal of human RFC3
Sequence YHLEVNPSDA GNSDRVVIQE MLKTVAQSQQ LETNSQRDFK VVLLTEVDKL
Predicted Reactivity Dog : 100 %, Guinea pig : 100 %, Horse : 100 %, Human : 100 %, Mouse : 90 %, Pig : 90 %, Rabbit : 90 %, Rat : 90 %, African clawed frog : 80 %, Bovine : 80 %, Chicken : 80 %
Characteristics This is a rabbit polyclonal antibody against RFC3. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name RFC3
Background The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kDa. RFC3 is the 38 kDa subunit. This subunit is essential for the interaction between the 140 kDa subunit and the core complex that consists of the 36, 37, and 40 kDa subunits.The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 38 kD subunit. This subunit is essential for the interaction between the 140 kD subunit and the core complex that consists of the 36, 37, and 40 kD subunits. Alternatively spliced transcript variants encoding distinct isoforms have been described.
Molecular Weight 40 kDa
Gene ID 5983
NCBI Accession NM_002915, NP_002906
UniProt P40938
Research Area Chromatin and Nuclear Signaling, DNA/RNA
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 356 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Replication Factor C (Activator 1) 3, 38kDa (RFC3) (N-Term) antibody WB Suggested Anti-RFC3 Antibody Titration: 0.2-1 ug/ml
Positive Control: HepG2 cell lysate
Background publications Zou, Liu, Elledge: "Replication protein A-mediated recruitment and activation of Rad17 complexes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 24, pp. 13827-32, 2003 (PubMed).

Validation Images
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