Nanog Homeobox (NANOG) (N-Term) antibody

Details for Product No. ABIN310961
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Antigen
Synonyms 2410002E02Rik, ENK, ecat4
Epitope
N-Term
(15), (15), (12), (11), (9), (7), (6), (6), (6), (5), (5), (5), (4), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity
Pig (Porcine), Cow (Bovine), Human
(161), (42), (20), (14), (3), (1)
Host
Rabbit
(121), (51), (12), (3)
Clonality
Polyclonal
Conjugate
Un-conjugated
(7), (6), (6), (5), (5), (5), (2), (2), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(146), (100), (40), (34), (31), (23), (18), (16), (10), (5), (1), (1)
Pubmed 1 reference available
Quantity 50 μg
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Catalog No. ABIN310961
289.00 $
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Immunogen Synthetic peptide directed towards the N terminal of human NANOG
Sequence ESSLTPVTCG PEENYPSLQM SSAEMPHAET VSPLPSSMDL LIQDSPDSST
Predicted Reactivity Bovine : 100 %, Human : 100 %, Pig : 100 %, Sheep : 100 %, Dog : 90 %
Characteristics This is a rabbit polyclonal antibody against NANOG. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name NANOG
Background NANOG is a new marker for testicular carcinoma in situ and germ cell tumors. Gene knockdown of Nanog promotes differentiation, thereby demonstrating a role for these factors in human embryonic stem cell self-renewal.
Molecular Weight 33 kDa
Gene ID 79923
NCBI Accession NP_079141, NM_024865
UniProt Q9H9S0
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 305 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Nanog Homeobox (NANOG) (N-Term) antibody WB Suggested Anti-NANOG Antibody Titration: 0.2-1 ug/ml
Positive Control: Jurkat cell lysate
Background publications Piestun, Kochupurakkal, Jacob-Hirsch et al.: "Nanog transforms NIH3T3 cells and targets cell-type restricted genes." in: Biochemical and biophysical research communications, Vol. 343, Issue 1, pp. 279-85, 2006 (PubMed).

Validation Images
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