RFT1 Homolog (S. Cerevisiae) (RFT1) (N-Term) antibody

Details for Product No. ABIN311263
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Antigen
Synonyms GB14370, CDG1N, D930025H04, RGD1562654
Epitope
N-Term
(9), (8), (2), (2), (1)
Reactivity
Rat (Rattus), Mouse (Murine), Dog (Canine), Cow (Bovine), Human
(14)
Host
Rabbit
(13), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(14), (8), (7), (2), (1), (1)
Pubmed 1 reference available
Quantity 50 μg
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Catalog No. ABIN311263
289.00 $
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Immunogen Synthetic peptide directed towards the N terminal of human RFT1
Sequence GTQRDWSQTL NLLWLTVPLG VFWSLFLGWI WLQLLEVPDP NVVPHYATGV
Predicted Reactivity Dog : 100 %, Human : 100 %, Bovine : 90 %, Chicken : 90 %, Horse : 90 %, Rabbit : 90 %, Rat : 90 %, Mouse : 80 %
Characteristics This is a rabbit polyclonal antibody against RFT1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name RFT1
Background N-glycosylation of proteins follows a highly conserved pathway that begins with the synthesis of a��Man(5)GlcNAc(2)-dolichylpyrophosphate (PP-Dol) intermediate on the cytoplasmic side of the endoplasmic reticulum (ER) membrane followed by the translocation of Man(5)GlcNAc (2)-PP-Dol to the luminal side of the ER membrane. RFT1 is the flippase enzyme that catalyzes this translocation.N-glycosylation of proteins follows a highly conserved pathway that begins with the synthesis of a Man(5)GlcNAc(2)-dolichylpyrophosphate (PP-Dol) intermediate on the cytoplasmic side of the endoplasmic reticulum (ER) membrane followed by the translocation of Man(5)GlcNAc (2)-PP-Dol to the luminal side of the ER membrane. RFT1 is the flippase enzyme that catalyzes this translocation (Helenius et al., 2002 [PubMed 11807558]).
Molecular Weight 60 kDa
Gene ID 91869
NCBI Accession NM_052859, NP_443091
UniProt Q96AA3
Research Area Signaling, Metabolism
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 541 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-RFT1 Homolog (S. Cerevisiae) (RFT1) (N-Term) antibody WB Suggested Anti-RFT1 Antibody Titration: 0.2-1 ug/ml
Positive Control: HepG2 cell lysate
Background publications Haeuptle, Pujol, Neupert et al.: "Human RFT1 deficiency leads to a disorder of N-linked glycosylation." in: American journal of human genetics, Vol. 82, Issue 3, pp. 600-6, 2008 (PubMed).

Validation Images
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