Sperm Associated Antigen 11B (SPAG11B) (N-Term) antibody

Details for Product No. ABIN311630
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Antigen
Synonyms Ep2c/h, EG546038, Spag11c/h, SPAG11B, EP2C, EDDM2B, EP2, EP2D, HE2, HE2C, SPAG11
Epitope
N-Term
(2), (1)
Reactivity
Human
(2), (1)
Host
Rabbit
(4)
Clonality
Polyclonal
Application
Western Blotting (WB)
(4), (1)
Pubmed 1 reference available
Quantity 50 µg
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Catalog No. ABIN311630
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Immunogen Synthetic peptide directed towards the N terminal of human SPAG11B
Sequence MRQRLLPSVT SLLLVALLFP GSSQARHVNH SATEALGELR ERAPGQGTNG
Predicted Reactivity Human : 100 %
Characteristics This is a rabbit polyclonal antibody against SPAG11B. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name SPAG11B
Background SPAG11B is one of several androgen-dependent, epididymis-specific secretory proteins. The specific functions of these proteins have not been determined, but they are thought to be involved in sperm maturation. Some of the isoforms contain regions of similarity to beta-defensins, a family of antimicrobial peptides.This gene encodes several androgen-dependent, epididymis-specific secretory proteins. The specific functions of these proteins have not been determined, but they are thought to be involved in sperm maturation. Some of the isoforms contain regions of similarity to beta-defensins, a family of antimicrobial peptides. The gene is located on chromosome 8p23 near the defensin gene cluster. Alternative splicing of this gene results in seven transcript variants encoding different isoforms. Two different N-terminal and five different C-terminal protein sequences are encoded by the splice variants. Two additional variants have been described, but their full length sequences have not been determined.
Molecular Weight 15 kDa
Gene ID 10407
NCBI Accession NM_058201, NP_478108
Research Area Immunology
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 133 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Sperm Associated Antigen 11B (SPAG11B) (N-Term) antibody WB Suggested Anti-SPAG11B Antibody Titration: 0.2-1 ug/ml
Positive Control: Human Muscle
Background publications Avellar, Honda, Hamil et al.: "Differential expression and antibacterial activity of epididymis protein 2 isoforms in the male reproductive tract of human and rhesus monkey (Macaca mulatta)." in: Biology of reproduction, Vol. 71, Issue 5, pp. 1453-60, 2004 (PubMed).

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