Finkel-Biskis-Reilly Murine Sarcoma Virus (FBR-MuSV) Ubiquitously Expressed (FAU) (Middle Region) antibody

Details for Product No. ABIN311722
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Antigen
Synonyms FAU1, Fub1, Fubi, MNSFbeta, RPS30, S30, asr1, Asr1, MGC73171, zgc:73171
Epitope
Middle Region
(10), (10), (8), (2), (2), (2), (2), (2), (1)
Reactivity
Mouse (Murine), Rat (Rattus), Pig (Porcine), Cow (Bovine), Xenopus laevis, Dog (Canine), Zebrafish (Danio rerio), Human
(50), (5), (3)
Host
Rabbit
(47), (3)
Clonality
Polyclonal
Conjugate
Un-conjugated
(4), (4), (4), (4), (4), (4)
Application
Western Blotting (WB)
(50), (35), (24), (10), (3), (2), (1)
Pubmed 1 reference available
Quantity 50 µg
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Catalog No. ABIN311722
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Immunogen Synthetic peptide directed towards the middle region of human FAU
Sequence VRGQTPKVAK QEKKKKKTGR AKRRMQYNRR FVNVVPTFGK KKGPNANS
Predicted Reactivity Bovine : 100 %, Dog : 100 %, Horse : 100 %, Human : 100 %, Mouse : 100 %, Pig : 100 %, Rat : 100 %, Zebrafish : 100 %
Characteristics This is a rabbit polyclonal antibody against FAU. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name FAU
Background FAU is a fusion protein consisting of the ubiquitin-like protein fubi at the N terminus and ribosomal protein S30 at the C terminus. It has been proposed that the fusion protein is post-translationally processed to generate free fubi and free ribosomal protein S30. Fubi is a member of the ubiquitin family, and ribosomal protein S30 belongs to the S30E family of ribosomal proteins. Whereas the function of fubi is currently unknown, ribosomal protein S30 is a component of the 40S subunit of the cytoplasmic ribosome.This gene is the cellular homolog of the fox sequence in the Finkel-Biskis-Reilly murine sarcoma virus (FBR-MuSV). It encodes a fusion protein consisting of the ubiquitin-like protein fubi at the N terminus and ribosomal protein S30 at the C terminus. It has been proposed that the fusion protein is post-translationally processed to generate free fubi and free ribosomal protein S30. Fubi is a member of the ubiquitin family, and ribosomal protein S30 belongs to the S30E family of ribosomal proteins. Whereas the function of fubi is currently unknown, ribosomal protein S30 is a component of the 40S subunit of the cytoplasmic ribosome. Pseudogenes derived from this gene are present in the genome. Similar to ribosomal protein S30, ribosomal proteins S27a and L40 are synthesized as fusion proteins with ubiquitin.
Molecular Weight 14 kDa
Gene ID 2197
NCBI Accession NM_001997, NP_001988
UniProt P62861
Research Area Proteolysis / Ubiquitin, DNA/RNA
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 133 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Finkel-Biskis-Reilly Murine Sarcoma Virus (FBR-MuSV) Ubiquitously Expressed (FAU) (Middle Region) antibody WB Suggested Anti-FAU Antibody Titration: 0.2-1 ug/ml
Positive Control: 721_B cell lysate
Background publications Yu, Ji, Doudna et al.: "Mass spectrometric analysis of the human 40S ribosomal subunit: native and HCV IRES-bound complexes." in: Protein science : a publication of the Protein Society, Vol. 14, Issue 6, pp. 1438-46, 2005 (PubMed).

Validation Images
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