CD20 (cytoplasmic) antibody

Antigen: CD20

Epitope: cytoplasmic

Clonality: Monoclonal (1412)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS)

Catalog no.: ABIN311765

Additional Information

Alternative name: CD20 (cytoplasmic)

Isotype: IgG2a, kappa  (Matching secondary antibodies)

Clone: 1412

Description: CD20 is a 33-37 kD four transmembrane spanning protein also known as B1 and Bp35. CD20 is expressed onpre-B-cells, resting and activated B cells (not plasma cells), some follicular dendritic cells, and at low levels on a T cellsubset. CD20 is heavily phosphorylated in activated B cells and malignant B cells. Homo-oligomeric complexes ofCD20 are thought to form Ca2+ conductive ion channels in the plasma membrane of B cells. The CD20 molecule isinvolved in B-cell activation and is associated with various Src family kinases (Lyn, Lck, Fyn), and exists in a complexwith MHC class I and II, CD53, CD81, and CD82. The mAb 1412 recognizes the cytoplasmic domain of the CD20molecule.

Application Details

Application Notes: Each lot of this antibody is quality control tested by immunofluorescentstaining with flow cytometric analysis. For immunofluorescent staining, thesuggested use of this reagent is 5 ul per million cells or 5 ul per 100 ul ofwhole blood. It is recommended that the reagent be titrated for optimalperformance for each application.* Alexa Fluor

Purification: The antibody was purified by affinity chromatography, and conjugated withAlexa Fluor 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor 488.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and0.2% (w/v) BSA (origin USA).

Storage: The antibody solution should be stored undiluted at 4 degrees C and protected from prolonged exposure to light. Do not freeze.

Restrictions: For Research Use only