TEK Tyrosine Kinase, Endothelial (TEK) antibody (Alexa Fluor 488)

Antigen: TEK Tyrosine Kinase, Endothelial (TEK)

Synonyms: TIE2, VMCM, TIE-2, VMCM1, CD202B, MGC139569, TEK, DKFZp469E064

Clonality: Monoclonal (33-1(Ab33))

Host: Mouse

Reactivity: Human

Conjugate: Alexa Fluor 488

Application: Flow Cytometry (FACS)

Catalog no.: ABIN311778

Additional Information

Alternative name: CD202b (Tie2/Tek)

Immunogen: Recombinant extracellular domain of human Tie2

Isotype: IgG1, kappa chain

Clone: 33-1(Ab33)

Description: CD202b is a 145 kD type I transmembrane protein, also known as Tie2 or TEK. It is a member of the receptortyrosine kinase (RTK) family of proteins and is expressed by endothelial cells and their progenitors, quiescenthematopoietic stem cells (HSCs), and a subpopulation of monocytes. Angiopoietin-1 (Ang-1) is an activator ofCD202b to promote, maintain, and stabilize mature vessels and to maintain HSCs in quiescent state. Ang-2 is anotherligand of CD202b, which is involved in postnatal angiogenesis and in antagonizing the effects of Ang-1. Tie2 binds toAng-4 as well.

Application Details

Application Notes: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 ul per million cells or 5 ul per 100 ul of wholeblood. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor

Purification: The antibody was purified by affinity chromatography, and conjugated withAlexa Fluor 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor 488 and unconjugated antibody.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and0.2% (w/v) BSA (origin USA).

Storage: The antibody solution should be stored undiluted at 4 degrees C and protected from prolonged exposure to light. Do not freeze.

Restrictions: For Research Use only