IL-8 antibody (Alexa Fluor 488)

Antigen: IL-8

Clonality: Monoclonal (E8N1)

Host: Mouse

Reactivity: Human

Conjugate: Alexa Fluor 488

Application: Intracellular Flow Cytometry (ICFC)

Catalog no.: ABIN311785

Additional Information

Isotype: IgG1, kappa chain

Clone: E8N1

Description: IL-8, also known as neutrophil chemotactic factor, neutrophil activating protein, and monocyte-derived neutrophilchemotactic factor, is a member of the alpha (C-X-C) subfamily of chemokines called CXCL8. In response toproinflammatory stimuli, IL-8 is produced by monocytes, macrophages, T cells, neutrophils, and fibroblasts. IL-8promotes neutrophil chemotaxis and degranulation. The 72 amino acid IL-8 is the predominant form secreted bymonocytes and lymphocytes. The E8N1 antibody recognizes the human IL-8 protein and has been shown to be usefulfor intracellular immunofluorescence flow cytometric analysis and as ELISA detection antibody.

Application Details

Application Notes: Each lot of this antibody is quality control tested by immunofluorescentstaining with flow cytometric analysis. For immunofluorescent staining, thesuggested use of this reagent is 5 ul per 106 cells in 100 ul volume. It isrecommended that the reagent be titrated for optimal performance for eachapplication.* Alexa Fluor

Purification: The antibody was purified by affinity chromatography, and conjugated withAlexa Fluor 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor 488.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide, 0.2%(w/v) BSA (USA origin).

Storage: The antibody solution should be stored undiluted at 4 degrees C and protected from prolonged exposure to light. Do not freeze.

Restrictions: For Research Use only