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Antibodies
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anti-SSEA-4 antibody (Alexa Fluor 488)
SSEA-4 antibody (Alexa Fluor 488)
| Antigen |
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| Clonality |
Monoclonal (MC-813-70) |
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Host
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Reactivity
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Conjugate
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Alexa Fluor 647 (2), FITC (2), PE (2), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), HRP (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Application
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| Catalog no. |
ABIN311787 |
| Quantity |
25 tests (Variants) |
| Price |
Product not available in this region.
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| Shipping to |
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Immunogen
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Human embryonic carcinoma cell line 2102Ep
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Isotype
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IgG3, kappa chain
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Clone
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MC-813-70
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Description
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The MC813-70 antibody reacts with a carbohydrate antigen which is named as Stage-Specific Embryonic Antigen-4(SSEA-4). It is a terminal structure of globo-series glycolipids (NeuAcalpha2-3Galbeta1-3GalNAcbeta1-R). SSEA-4 is expressedon human teratocarcinoma cells (EC), embryonic stem cells (ESC), embryonic germ cells (EG), and preimplantationembryos, subset of mesenchymal stem cells, erythrocytes, and erythromyeloid leukemia cell line K562. Theexpression of SSEA-3 on human ESC is downregulated upon differentiation. SSEA-4 is also expressed by mouse onunfertilized eggs and preimplantation to early cleaved embryos.
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Application Notes
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 ul per million cells or 5 ul per 100 ul of wholeblood. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor
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Purification
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The antibody was purified by affinity chromatography, and conjugated withAlexa Fluor 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor 488.
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Buffer
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Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and0.2% (w/v) BSA (origin USA).
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Storage
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The antibody solution should be stored undiluted at 4 degrees C and protected from prolonged exposure to light. Do not freeze.
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Restrictions
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For Research Use only
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Alternatives for antigen "SSEA-4", type "Antibodies"
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Hosts
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Rabbit (20), Mouse (6)
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Reactivities
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Human (26), Mouse (Murine) (21), Chicken (5), Dog (Canine) (5), Monkey (5), Rabbit (5), Rat (Rattus) (1)
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Applications
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Flow Cytometry (FACS) (24), Immunofluorescence (IF) (21), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoprecipitation (IP) (2), Western Blotting (WB) (2), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (IHC) (1)
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Conjugates
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Alexa Fluor 647 (2), FITC (2), PE (2), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), HRP (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Alternatives 
