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CD3 (CD3E) antibody (Alexa Fluor 488)

Antigen

CD3 (CD3E)
Synonyms T3E, TCRE, FLJ18683
Clonality Monoclonal (1F4)
Host
Alternatives

Mouse
Reactivity
Alternatives

Rat (Rattus)
Conjugate
Alternatives Alexa Fluor 488
Application
Alternatives Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)
Catalog no. ABIN311794
Quantity 100 ug  (Variants)
Price Product not available in this region.
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anti-CD3 (CD3E) antibody (Alexa Fluor 488)

Additional Information
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Alternative name CD3
Immunogen F344 rat spleen cells stimulated with PMA and calcium ionophore
Isotype IgM, kappa chain
Clone 1F4
Description CD3 is a complex composed of delta, gamma, epsilon, and zeta chains. They are 20-25 kD members of the immunoglobulin superfamilyand associated with the T cell receptor (TCR). CD3 is expressed on thymocytes, peripheral T cells, some NK-T cellsand dendritic epidermal T cells. CD3 is involved in antigen recognition, signal transduction, and T cell activation.Immobilized 1F4 antibody can induce T cell proliferation in vitro.

Application Details
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Application Notes Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is approx. 1.0 ug per 106 cells in 100 ul volume. It isrecommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor
Purification The antibody was conjugated with Alexa Fluor 488 under optimalconditions, and is at >85% purity. The solution is free of unconjugated AlexaFluor 488.
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Storage The antibody solution should be stored undiluted at 4 degrees C and protected from prolonged exposure to light. Do not freeze.
Restrictions For Research Use only

Alternatives
Application Details Images Variants back to top

Alternatives for antigen "CD3 (CD3E)", type "Antibodies"
Hosts Mouse (310), Rat (55), Rabbit (51), Hamster (38)
Reactivities Human (293), Mouse (Murine) (105), Rat (Rattus) (43), Dog (Canine) (30), Pig (Porcine) (26), Chicken (18), Cow (Bovine) (17), Monkey (15), Primate (9), Bird (Avian) (4), Cat (Feline) (4), Hamster (3), Horse (Equine) (3), Primates (3), Rhesus Monkey (2), Rabbit (1)
Applications Flow Cytometry (FACS) (354), Immunohistochemistry (Frozen Sections) (IHC (fro)) (92), Immunoprecipitation (IP) (66), Functional Studies (Func) (49), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (49), Western Blotting (WB) (42), Immunofluorescence (IF) (32), ELISA (19), Immunohistochemistry (IHC) (17), Blocking Antibody (Inhibition) (10), Enzyme Immunoassay (EIA) (7), Immunocytochemistry (ICC) (6), Cytotoxicity Test (CyTox) (2), Dot Blot (Dot) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Radioimmunoassay (RIA) (2), Immunoelectron Microscopy (IEM) (1)
Conjugates FITC (76), Biotin (46), PE (39), APC (24), RPE (10), SPRD (7), Cy5 (6), Alexa Fluor 488 (4), Cy5,PE (4), DY647 (3), PE,Cy5 (3), PE,Cy7 (3), APC,Cy7 (2), Alexa Fluor 647 (2), Cy7,PE (2), PE,Cy5.5 (2), PE,DY647 (2), PE-Dyomics 647 (2), PerCP (2), RPE,Cy5 (2), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 555 (1), Alexa Fluor 700 (1), Cy3 (1), Cy5.5 (1), Cy7 (1), Gold (1), HRP (1), PE,Cy3 (1), Pacific Blue (1), RBITC (1), RPE,Cy7 (1)
Epitopes AA 156-168 (4), AA 196-206 (1), C-Term (1)