CD8a antibody (Alexa Fluor 488)

Antigen: CD8a

Clonality: Monoclonal

Host: Mouse

Reactivity: Rat (Rattus)

Conjugate: Alexa Fluor 488

Application: Flow Cytometry (FACS)

Catalog no.: ABIN311799

Additional Information

Immunogen: High molecular weight glycoproteins from rat thymocytes

Isotype: IgG1, kappa

Description: CD8a is a 32 kD glycoprotein also known as T8, Lyt2, Ly-2, and CD8alpha. CD8a is a member of the immunoglobulinsuperfamily expressed on most thymocytes, subset of mature T cells, most NK cells, macrophages, and someactivated CD4+ T cells (not resting). CD8a forms heterodimers with the CD8beta chain (CD8b) on the surface of mostthymocytes, while mature peripheral T lymphocytes express almost exclusively the CD8 alpha beta heterodimer. Intestinalintraepithelial lymphocytes express CD8a without CD8b. CD8 is an antigen co-receptor on T cells that interacts withMHC class I on antigen-presenting cells or epithelial cells. CD8 participates in T cell activation through its associationwith the T cell receptor complex and protein tyrosine kinase lck (p56lck). The OX-8 antibody has been reported topartially block T cell responses, including mixed lymphocyte reactions and cytotoxic T cell responses, and to inducemacrophage activation.

Application Details

Application Notes: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is approx. 0.5 ug per 106 cells in 100 ul volume. It isrecommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor

Purification: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor 488 under optimalconditions. The solution is free of unconjugated Alexa Fluor 488.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Storage: The antibody solution should be stored undiluted at 4 degrees C and protected from prolonged exposure to light. Do notfreeze.

Restrictions: For Research Use only

Publications

Publications: Torres-Nagel, Kraus, Brown et al.: "Differential thymus dependence of rat CD8 isoform expression." in: European journal of immunology, Vol. 22, Issue 11, pp. 2841-8, 1992 (PubMed).

Mason, Arthur, Dallman et al.: "Functions of rat T-lymphocyte subsets isolated by means of monoclonal antibodies." in: Immunological reviews, Vol. 74, pp. 57-82, 1983 (PubMed).

Barclay: "The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues." in: Immunology, Vol. 42, Issue 4, pp. 593-600, 1981 (PubMed).

Wallgren, Karlsson-Parra, Korsgren: "The main infiltrating cell in xenograft rejection is a CD4+ macrophage and not a T lymphocyte." in: Transplantation, Vol. 60, Issue 6, pp. 594-601, 1995 (PubMed).

Hirji, Lin, Befus: "A novel CD8 molecule expressed by alveolar and peritoneal macrophages stimulates nitric oxide production." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 4, pp. 1833-40, 1997 (PubMed).

Mitnacht, Bischof, Torres-Nagel et al.: "Opposite CD4/CD8 lineage decisions of CD4+8+ mouse and rat thymocytes to equivalent triggering signals: correlation with thymic expression of a truncated CD8 alpha chain in mice but not rats." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 2, pp. 700-7, 1998 (PubMed).