Add to Basket
Order hotline:
+1 404 474 4654
+1 888 205 9894 (TF)

Histone H3 (pSer28) antibody (Alexa Fluor 488)

Antigen

Histone H3
Synonyms
H3t, H3.4, H3/g, H3FT, MGC126886, MGC126888, Hist1, H3.2-221, DmelCG31613, CG31613, h3.2, h3/m, h3f2, h3fm, hist1h3g, H3F3, H3.3A, H3F3B, MGC87782, MGC87783, H3.3B, H3F3A, h3f3a, MGC52829, MGC86731, M ... show more
Epitope
Alternatives

pSer28
Clonality Polyclonal
Host
Reactivity
Alternatives

Human
Conjugate
Alternatives Alexa Fluor 488
Application
Alternatives Flow Cytometry (FACS)
Catalog no. ABIN311804
Quantity 25 tests
Price Product not available in this region.
Shipping to

Additional Information
Application Details Alternatives back to top

Alternative name Histone H3-Phosphorylated (Ser28)
Immunogen Synthetic peptide conjugated to KLH, corresponding to amino acids 23-35 of Human Histone H3.
Isotype IgG2a, kappa chain
Clone HTA28
Description H3 is a core component of the nucleosome that serves to wrap and compact DNA into chromatin. Histones therefore,limit the accessibility of DNA, providing mechanisms for transcription regulation, DNA repair and replication andchromosomal stability. During mitosis, H3 is phosphorylated at serine 28. This phosphorylation coincides withchromosome condensation initiated at prophase and disappears at late anaphase. H3 has been demonstrated to bephosphorylated by the action of MLTK-alpha (mixed linage kinase-like mitogen activated protein triple kinase alpha) inresponse to ultraviolet B light and epidermal growth factor, as well as Aurora-B during mitosis.
Synonyms H3t, H3.4, H3/g, H3FT, MGC126886, MGC126888, Hist1, H3.2-221, DmelCG31613, CG31613, h3.2, h3/m, h3f2, h3fm, hist1h3g, H3F3, H3.3A, H3F3B, MGC87782, MGC87783, H3.3B, H3F3A, h3f3a, MGC52829, MGC86731, MGC165454, zgc:56193, zgc:86731, wu:fb58e10, FLJ35099, FLJ56780, FLJ56787, MGC131639, MGC89877, HIST1H3A

Application Details
Alternatives back to top

Application Notes Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 ul per million cells or 5 ul per 100 ul of wholeblood. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor
Purification The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor 488 under optimalconditions. The solution is free of unconjugated Alexa Fluor 488.
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Storage The antibody solution should be stored undiluted at 4 degrees C and protected from prolonged exposure to light. Do notfreeze.
Restrictions For Research Use only

Alternatives
Application Details back to top

Alternatives for antigen "Histone H3", type "Antibodies"
Hosts Rabbit (137), Mouse (15), Sheep (4)
Reactivities Human (145), Rat (Rattus) (74), Mouse (Murine) (73), Cow (Bovine) (23), Pig (Porcine) (19), Rabbit (19), Fruit Fly (Drosophila melanogaster) (17), Dog (Canine) (7), Chicken (4), Horse (Equine) (3), Sheep (Ovine) (3), Guinea Pig (1), Tetrahymena (1)
Applications Western Blotting (WB) (96), Immunofluorescence (IF) (75), Flow Cytometry (FACS) (49), ELISA (43), Enzyme Immunoassay (EIA) (29), Dot Blot (Dot) (23), Immunohistochemistry (IHC) (21), Chromatin Immunoprecipitation (ChiP) (19), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (16), Immunoprecipitation (IP) (11), Immunocytochemistry (ICC) (7), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
Conjugates Alexa Fluor 488 (8), Alexa Fluor 350 (7), Alexa Fluor 555 (7), Alexa Fluor 647 (7), PE,Cy5.5 (7), Agarose Beads (1), Alexa Flour 488 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1), RBITC (1)
Epitopes pSer10 (23), pSer28 (17), pThr3 (12), pThr11 (6), 3meLys79 (2), Mono Methyl Lysine 4 (2), N-Term (2), Tri Methyl Lysine 4 (2), 2meArg17 (1), 2meLys27 (1), 2meLys27, 3meLys27 (1), 2meLys36 (1), 2meLys4 (1), 2meLys79 (1), 3meLys27 (1), 3meLys27, pSer10 (1), 3meLys4 (1), 3meLys9 (1), 3meLys9, pSer10 (1), Ab-23 (1), Ab-3 (1), Acetyl-Lys14 (1), Acetyl-Lys18 (1), Acetyl-Lys23 (1), Acetyl-Lys27 (1), Acetylated (1), Arg2 (1), acLys9 (1), acLys9, pSer10 (1), acetyl-Lys9 (1), meLys27 (1), meLys36 (1), meLys4 (1), meLys79 (1), meLys9 (1)