Panendothelial Cell Antigen antibody (Alexa Fluor 488)

Antigen: Panendothelial Cell Antigen

Clonality: Monoclonal (MECA-32)

Host: Rat

Reactivity: Mouse (Murine)

Conjugate: Alexa Fluor 488

Application: Flow Cytometry (FACS)

Catalog no.: ABIN311807

Additional Information

Immunogen: Mouse lymph node stromal cells

Isotype: IgG2a, kappa chain

Clone: MECA-32

Description: MECA-32 is a 50-55 kD homodimer glycoprotein primarily expressed on most endothelial cells in the embryonic andthe adult mouse tissues, except in the brain and in skeletal and cardiac muscle, where it has more restricteddistribution. In skeletal and cardiac muscle, the MECA-32 antigen is confined in small arterioles and venules. But insome conditions, such as inflammation or cardiac transplantation, MECA-32 expression can be induced on somenegative-cardiac endothelial cells. In the brain, its expression occurs only in the circumventricular organs and theneurohemal tissues. During embryonic development, MECA-32 antigen is expressed on the brain vasculature up today 16 of gestation, and disappears at day 17 and after.

Application Details

Application Notes: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is approx. 1.0 ug per 106 cells in 100 ul volume. It isrecommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor

Purification: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor 488 under optimalconditions. The solution is free of unconjugated Alexa Fluor 488.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Storage: The antibody solution should be stored undiluted at 4 degrees C and protected from prolonged exposure to light. Do notfreeze.

Restrictions: For Research Use only