SSEA-1 antibody (Alexa Fluor 488)

Antigen: SSEA-1

Clonality: Monoclonal (MC-480)

Host: Mouse

Reactivity: Mouse (Murine), Human

Conjugate: Alexa Fluor 488

Application: Flow Cytometry (FACS)

Catalog no.: ABIN311811

Additional Information

Immunogen: Mouse F9 Teratocarcinoma Stem Cells (X-irradiated)Reactivity: MouseCross-

Isotype: IgM, kappa chain

Clone: MC-480

Description: The MC-480 antibody reacts with mouse and human Stage-Specific Embryonic Antigen-1 (SSEA-1). It is a Lewisblood group related carbohydrate antigen known as X-hapten, Lewis X, 3-FAL, 3-fucosyl-N-acetyllactosamine, orCD15. The expression pattern of SSEA-1 antigen is different from human and mouse. In mouse, SSEA-1 isexpressed on embryonic stem cells (ES), embryonal carcinoma cells (EC), 8-cell to blastocyst embryos, and a subsetof embryonic inner cell mass. The expression on murine ES cells is decreased upon differentiation. In human,however, SSEA-1 is not found on undifferentiated ES cells, but its expression is upregulated along with differentiation.CD15 is highly expressed on adult human granulocytes. It has been reported that SSEA-1 plays a role in celladhesion and regulation of cell differentiation.

Application Details

Application Notes: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 ul per million cells or 5 ul per 100 ul of wholeblood. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor

Purification: The antibody was conjugated with Alexa Fluor 488 under optimalconditions, and is at >85% purity. The solution is free of unconjugated AlexaFluor 488.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and0.2% (w/v) BSA (origin USA).

Storage: The antibody solution should be stored undiluted at 4 degrees C and protected from prolonged exposure to light. Do not freeze.

Restrictions: For Research Use only