Chemokine (C-C Motif) Receptor 3 (CCR3) antibody (Alexa Fluor 647)

Antigen: Chemokine (C-C Motif) Receptor 3 (CCR3)

Synonyms: CKR3, CD193, CMKBR3, CC-CKR-3, MGC102841

Clonality: Monoclonal (5E8)

Host: Mouse

Reactivity: Human

Conjugate: Alexa Fluor 647

Application: Flow Cytometry (FACS)

Catalog no.: ABIN311832

Additional Information

Alternative name: CD193 (CCR3)

Isotype: IgG2b, kappa chain

Clone: 5E8

Description: CC-chemokine receptor 3 (CCR3), also known as CC CKR3, MIP1-alpha receptor like-2, eotaxin receptor, andCD193, is a member of G protein-coupled seven transmembrane receptors family. It binds to the CC chemokineseotaxin, eotaxin-2, eotaxin-3 with high affinity. CCR3 has also been reported to bind RANTES, MCP-3, and MCP-4with low affinity. CCR3 receptor is expressed on human eosinophils, basophils, mast cells, mononuclear phagocytes,platelets, CD34+ hematopoietic progenitor cells, Th2-like lymphocytes, and keratinocytes. CCR3 is thought to play arole in allergic diseases such as bronchial asthma and allergic rhinitis. CCR3 is a co-receptor for HIV-1 and -2, andthe binding of eotaxin with CCR3 has been shown to inhibit HIV infection in some cell types.

Application Details

Application Notes: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is approx. 1.0 ug per 106 cells in 100 ul volume or 100 ul ofwhole blood. It is recommended that the reagent be titrated for optimal performance for each application. Additional reported applications (for the relevant formats) include: The 5E8 antibody is useful for immunofluorescentstaining and flow cytometric analysis of CCR3 expression.

Purification: The antibody was purified by affinity chromatography, and conjugated withAlexa Fluor 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor 647.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and0.2% (w/v) BSA (origin USA).

Storage: The antibody solution should be stored undiluted at 4 degrees C and protected from prolonged exposure to light. Do not freeze.

Restrictions: For Research Use only