CD273 (B7-DC, PD-L2) antibody (Alexa Fluor 647)

Antigen: CD273 (B7-DC, PD-L2)

Clonality: Monoclonal (24F-10C12)

Host: Mouse

Reactivity: Human

Conjugate: Alexa Fluor 647

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Functional Studies (Func)

Catalog no.: ABIN311844

Additional Information

Immunogen: Human PD-L2 cDNA

Isotype: IgG2a, kappa chain

Clone: 24F-10C12

Description: CD273, known as B7-DC, is also called programmed death ligand 2 (PDL2). This ligand is a 25 kD type Itransmembrane protein and a member of B7 family within the immunoglobulin receptor superfamily and is expressedon a subset of dendritic cells, liver and a small subset of macrophages as well as a few transformed cell lines. CD273has been reported to be stimulatory on dendritic cells when cross-linked and to inhibit T cell activation upon engagingthe PD-1 receptor. CD273 has also been reported to bind to an alternative receptor and to mediate T cell activationthrough such non-PD1 mediated interactions.

Application Details

Application Notes: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 ul per million cells or 5 ul per 100 ul of wholeblood. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor

Purification: The antibody was purified by affinity chromatography, and conjugated withAlexa Fluor 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor 647 and unconjugated antibody.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and0.2% (w/v) BSA (origin USA).

Storage: The antibody solution should be stored undiluted at 4 degrees C and protected from prolonged exposure to light. Do not freeze.

Restrictions: For Research Use only