Programmed Cell Death 1 (PDCD1) antibody (Alexa Fluor 647)

Antigen: Programmed Cell Death 1 (PDCD1)

Synonyms: PD1, CD279, SLEB2, hPD-1, hPD-l

Clonality: Monoclonal (EH12-2H7)

Host: Mouse

Reactivity: Human

Conjugate: Alexa Fluor 647

Application: Flow Cytometry (FACS)

Catalog no.: ABIN311846

Additional Information

Alternative name: CD279 (PD-1)

Isotype: IgG1, kappa chain

Clone: EH12-2H7

Description: The program death 1 (PD-1) receptor CD279, a 55 kDa member of the immunoglobulin superfamily. CD279/PD-1contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic region and plays a key role inperipheral tolerance and autoimmune disease. CD279/PD-1 is expressed predominantly on activated T cells, B cellsand and myeloid cells. PD-L1 (B7-H1) and PD-L2 (B7-DC)are ligands of CD279/PD-1 and are members of the B7gene family. Evidence reported to date suggests overlapping functions for these two PD-1 ligands and theirconstitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. Interaction ofCD279:PD-Ligands results in inhibition of T cell proliferation and cytokine secretion.

Application Details

Application Notes: Each lot of this antibody is quality control tested by immunofluorescentstaining with flow cytometric analysis. For immunofluorescent staining, thesuggested use of this reagent is 5 ul per million cells or 5 ul per 100 ul ofwhole blood. It is recommended that the reagent be titrated for optimalperformance for each application.* Alexa Fluor

Purification: The antibody was purified by affinity chromatography, and conjugated withAlexa Fluor 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor 647 and unconjugated antibody.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and0.2% (w/v) BSA (origin USA).

Storage: The antibody solution should be stored undiluted at 4 degrees C and protected from prolonged exposure to light. Do not freeze.

Restrictions: For Research Use only