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Antibodies
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anti-Intercellular Adhesion Molecule 1 (ICAM1) antibody (Alexa Fluor 647)
Intercellular Adhesion Molecule 1 (ICAM1) antibody (Alexa Fluor 647)
| Antigen |
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| Synonyms |
BB2, CD54, P3.58, Ly-47, Icam-1, MALA-2, MGC6195, ICAM-1, ICAM1, ICAM |
| Clonality |
Monoclonal (HCD54) |
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Host
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Reactivity
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Human (339), Mouse (Murine) (141), Rat (Rattus) (97), Cow (Bovine) (56), Pig (Porcine) (44), Rabbit (43), Dog (Canine) (38), Sheep (Ovine) (18), Horse (Equine) (16), Chicken (4), Guinea Pig (2), Fish (1)
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Conjugate
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Biotin (42), FITC (38), PE (29), Alexa Fluor 488 (8), Alexa Fluor 350 (7), Alexa Fluor 555 (7), Alexa Fluor 647 (7), Cy3 (7), Cy5 (7), Cy5.5 (7), Cy7 (7), Gold (7), HRP (7), PE,Cy3 (7), PE,Cy5 (7), PE,Cy5.5 (7), PE,Cy7 (7), RBITC (7), RPE (7), APC (6), Alexa Flour 488 (6), Alexa Fluor 700 (1), Pacific Blue (1), PerCP (1)
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Application
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Flow Cytometry (FACS) (328), Immunofluorescence (IF) (135), Western Blotting (WB) (126), Immunoprecipitation (IP) (81), Immunohistochemistry (Frozen Sections) (IHC (fro)) (76), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (62), ELISA (61), Immunohistochemistry (IHC) (20), Functional Studies (Func) (19), Enzyme Immunoassay (EIA) (14), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (12), Immunocytochemistry (ICC) (11), Blocking Antibody (Inhibition) (10), Immunoelectron Microscopy (IEM) (7), Blocking Antibody (Inhibition) (6), Immunohistochemistry (Fixed) (IHC (fx)) (5), ELISA (Detection) (2)
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| Catalog no. |
ABIN311864 |
| Quantity |
100 tests |
| Price |
Product not available in this region.
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| Shipping to |
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Alternative name
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CD54
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Isotype
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IgG1, kappa chain
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Clone
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HCD54
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Description
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CD54 is a 85-110 kD type I transmembrane protein also known as ICAM-1. It is expressed on activated endothelialcells, high endothelial venules, T and B cells, monocytes/macrophages, granulocytes, and dendritic cells. Theexpression of ICAM-1 on the cell surface is potently upregulated by activation, a soluble form of ICAM-1 can bereleased from the cell surface. CD54 plays a role in cellular adhesion and is involved in inflammation and leukocyteextravasation. CD54 has also been shown to be the major cellular receptor for rhinovirus. ICAM-1 binds toCD11a/CD18 (LFA-1), CD11b/CD18 (Mac-1), CD11c/CD18 (p150, 95) as well as hyaluronan and fibrinogen.
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Application Notes
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Each lot of this antibody is quality control tested by immunofluorescentstaining with flow cytometric analysis. For immunofluorescent staining, thesuggested use of this reagent is 5 ul per million cells or 5 ul per 100 ul ofwhole blood. It is recommended that the reagent be titrated for optimalperformance for each application.* Alexa Fluor
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Purification
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The antibody was purified by affinity chromatography, and conjugated withAlexa Fluor 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor 647.
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Buffer
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Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and0.2% (w/v) BSA (origin USA).
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Storage
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The antibody solution should be stored undiluted at 4 degrees C and protected from prolonged exposure to light. Do not freeze.
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Restrictions
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For Research Use only
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Alternatives for antigen "Intercellular Adhesion Molecule 1 (ICAM1)", type "Antibodies"
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Hosts
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Mouse (190), Rabbit (182), Rat (53), Hamster (4), Sheep (1)
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Reactivities
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Human (339), Mouse (Murine) (141), Rat (Rattus) (97), Cow (Bovine) (56), Pig (Porcine) (44), Rabbit (43), Dog (Canine) (38), Sheep (Ovine) (18), Horse (Equine) (16), Chicken (4), Guinea Pig (2), Fish (1)
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Applications
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Flow Cytometry (FACS) (328), Immunofluorescence (IF) (135), Western Blotting (WB) (126), Immunoprecipitation (IP) (81), Immunohistochemistry (Frozen Sections) (IHC (fro)) (76), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (62), ELISA (61), Immunohistochemistry (IHC) (20), Functional Studies (Func) (19), Enzyme Immunoassay (EIA) (14), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (12), Immunocytochemistry (ICC) (11), Blocking Antibody (Inhibition) (10), Immunoelectron Microscopy (IEM) (7), Blocking Antibody (Inhibition) (6), Immunohistochemistry (Fixed) (IHC (fx)) (5), ELISA (Detection) (2)
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Conjugates
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Biotin (42), FITC (38), PE (29), Alexa Fluor 488 (8), Alexa Fluor 350 (7), Alexa Fluor 555 (7), Alexa Fluor 647 (7), Cy3 (7), Cy5 (7), Cy5.5 (7), Cy7 (7), Gold (7), HRP (7), PE,Cy3 (7), PE,Cy5 (7), PE,Cy5.5 (7), PE,Cy7 (7), RBITC (7), RPE (7), APC (6), Alexa Flour 488 (6), Alexa Fluor 700 (1), Pacific Blue (1), PerCP (1)
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Epitopes
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pTyr512 (9), C-Term (2), AA 253-267 (1), Domain D1 (1)
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