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Antibodies
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anti-CD97 (CD97) antibody (Alexa Fluor 647)
CD97 (CD97) antibody (Alexa Fluor 647)
| Antigen |
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| Synonyms |
TM7LN1 |
| Clonality |
Monoclonal (VIM3b) |
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Host
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Reactivity
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Conjugate
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FITC (6), APC (4), PE (4), RPE (2), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), HRP (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Application
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Flow Cytometry (FACS) (46), Immunofluorescence (IF) (22), Western Blotting (WB) (17), Immunoprecipitation (IP) (14), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (8), ELISA (5), Enzyme Immunoassay (EIA) (5), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), ELISA (Detection) (1), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
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| Catalog no. |
ABIN311879 |
| Quantity |
25 tests (Variants) |
| Price |
Product not available in this region.
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| Shipping to |
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Alternative name
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CD97
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Isotype
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IgG1, kappa chain
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Clone
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VIM3b
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Description
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CD97 is a seven-span transmembrane protein belonging to the secretin receptor superfamily, the G-protein-coupledreceptor superfamily, and the EGF-TM7 family with molecular weights of 74 kD, 80 kD, and 86 kD. It is expressed onmonocytes/macrophages, granulocytes, dendritic cells, and smooth muscle cells. The expression of CD97 on restingT- and B-lymphocytes is at low level, but is rapidly upregulated upon activation. CD97 binds to CD55(decay-accelerating factor), chondroitin sulfate, integrin a5b1, and a3b1. CD97 has been shown to mediate celladhesion and co-stimulation of T cell proliferation.
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Application Notes
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 ul per million cells or 5 ul per 100 ul of wholeblood. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor
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Purification
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The antibody was purified by affinity chromatography, and conjugated withAlexa Fluor 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor 647.
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Buffer
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Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and0.2% (w/v) BSA (origin USA).
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Storage
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The antibody solution should be stored undiluted at 4 degrees C and protected from prolonged exposure to light. Do not freeze.
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Restrictions
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For Research Use only
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Alternatives for antigen "CD97 (CD97)", type "Antibodies"
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Hosts
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Mouse (32), Rabbit (29)
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Reactivities
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Human (58), Mouse (Murine) (25), Rat (Rattus) (20), Cow (Bovine) (15), Dog (Canine) (15), Pig (Porcine) (15), Monkey (2), Chimpanzee (1)
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Applications
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Flow Cytometry (FACS) (46), Immunofluorescence (IF) (22), Western Blotting (WB) (17), Immunoprecipitation (IP) (14), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (8), ELISA (5), Enzyme Immunoassay (EIA) (5), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), ELISA (Detection) (1), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
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Conjugates
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FITC (6), APC (4), PE (4), RPE (2), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), HRP (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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AA 220-270 (2), AA 1-512 (1)
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Alternatives 
