TCL1 antibody (Alexa Fluor 647)

Antigen: TCL1

Clonality: Monoclonal (1-21)

Host: Mouse

Reactivity: Human

Conjugate: Alexa Fluor 647

Application: Intracellular Flow Cytometry (ICFC)

Catalog no.: ABIN311908

Additional Information

Immunogen: outer alpha-loop region of TCL1 peptide

Isotype: IgG2b, kappa chain

Clone: 1-21

Description: TCL1, also known as T-cell leukemia/lymphoma protein 1A, protein p14 TCL1 and oncogene TCL-1, is a 14kDbeta-barrel protein that was originally identified on the basis of its association with T-cell prolymphocytic leukemia(T-PLL). Activated by the T-cell receptor enhancer through inv(14)(q11,q32.1) or t(14,14)(q11,q32.1), TCL1 isnormally expressed in immature cortical thymocytes, activated peripheral T-cells, pro-B cells, and naive mantle zoneB cells of peripheral lymphoid tissues, but not in post-germinal center (GC) memory B cells and plasma cells. Akt andTCL1 interact via Akt&rsquo,s pleckstrin homology domain to form hetero-oligomers at the leaflet of the inner plasmamembrane stimulating the protein kinase C-mitogen activated protein kinase-extracellular signal-related kinase(PKC-MAPK-ERK) signal transduction pathway. TCL1 also inhibits activation-induced cell death in T-cells by blockingPKC&theta,. In addition to T-PLL dysregulation is implicated in such B-cell neoplasias as lymphoblastic lymphoma,chronic lymphocytic leukemia (CLL) and Burkitt lymphoma.

Application Details

Application Notes: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 ul per million cells or 5 ul per 100 ul of wholeblood. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor

Purification: The antibody was purified by affinity chromatography, and conjugated withAlexa Fluor 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor 647.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and0.2% (w/v) BSA (origin USA).

Storage: The antibody solution should be stored undiluted at 4 degrees C and protected from prolonged exposure to light. Do not freeze.

Restrictions: For Research Use only