CD1d (CD1.1, Ly-38) antibody (Alexa Fluor 647)

Antigen: CD1d (CD1.1, Ly-38)

Clonality: Monoclonal (1B1)

Host: Rat

Reactivity: Mouse (Murine)

Conjugate: Alexa Fluor 647

Application: Flow Cytometry (FACS)

Catalog no.: ABIN311927

Additional Information

Immunogen: Mouse Cd1.1 cDNA-transfected RMA-S mouse T lymphom

Isotype: IgG2b-kappa

Clone: 1B1

Description: CD1d, known as CD1.1 and Ly-38, is a 48 kD type I membrane glycoprotein with structural similarities to MHC class Iand is non-covalently associated with beta2-microglobulin. In humans, CD1 family consists of group I proteins (CD1a,CD1b, and CD1c), group II (CD1d), and group III (CD1e). But CD1d is the only CD1 molecule has been found inmouse. Mouse CD1d has broad tissue distribution, and is found on leukocytes, dendritic cells, epithelial cells, andthymocytes. CD1d plays a role in non-peptide glycolipid antigen presentation to CD1d-restricted T cells. It has beenshown that PKCdelta is a critical regulator of CD1d-mediated antigen presentation.

Application Details

Application Notes: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is approx. 0.5 ug per 106 cells in 100 ul volume. It isrecommended that the reagent be titrated for optimal performance for each application. Additional reported applications (for the relevant formats) include: immunoprecipitation, immunohistochemisticalstaining, and blocking function1.

Purification: The antibody was purified by affinity chromatography, and conjugated withAlexa Fluor 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor 647 and unconjugated antibody.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Storage: The antibody solution should be stored undiluted at 4 degrees C and protected from prolonged exposure to light. Do not freeze.

Restrictions: For Research Use only