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Antibodies
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anti-CD8b antibody (Alexa Fluor 647)
CD8b antibody (Alexa Fluor 647)
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| Clonality |
Monoclonal (YTS156-7-7) |
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Host
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Reactivity
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Conjugate
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Application
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| Catalog no. |
ABIN311954 |
| Quantity |
100 ug (Variants) |
| Price |
Product not available in this region.
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| Shipping to |
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Alternative name
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CD8b (Ly-3)
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Immunogen
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mouse thymocytes
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Isotype
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IgG2b, kappa chain
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Clone
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YTS156-7-7
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Description
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CD8b is the 32 kD beta chain of CD8, also known as Lyt-3 or Ly-3. It is a member of the Ig superfamily expressed as aheterodimer with the CD8alpha chain on a subset of MHC class I-restricted T cells and most thymocytes. CD8 is aco-receptor for the TCR complex involved in T cell activation.
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Application Notes
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is approx. 0.25 ug per 106 cells in 100 ul volume. It isrecommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor
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Purification
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The antibody was purified by affinity chromatography, and conjugated withAlexa Fluor 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor 647.
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Buffer
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Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
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Storage
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The antibody solution should be stored undiluted at 4 degrees C and protected from prolonged exposure to light. Do not freeze.
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Restrictions
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For Research Use only
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Publications
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McNicol, Bendle, Holler et al.: "CD8alpha/alpha homodimers fail to function as co-receptor for a CD8-dependent TCR." in: European journal of immunology, Vol. 37, Issue 6, pp. 1634-41, 2007 (PubMed).
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Alternatives for antigen "CD8b", type "Antibodies"
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Hosts
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Mouse (51), Rat (25), Rabbit (5)
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Reactivities
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Mouse (Murine) (27), Rat (Rattus) (26), Chicken (12), Cow (Bovine) (6), Bird (Avian) (4), Human (4), Cat (Feline) (2), Dog (Canine) (1)
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Applications
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Flow Cytometry (FACS) (74), Immunoprecipitation (IP) (26), Cytotoxicity Test (CyTox) (16), Western Blotting (WB) (16), Immunohistochemistry (Frozen Sections) (IHC (fro)) (9), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), Functional Studies (Func) (1), Immunocytochemistry (ICC) (1)
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Conjugates
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FITC (15), PE (15), Biotin (12), APC (3), RPE (3), Cy5,PE (2)
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Alternatives 
