Folate Receptor 4 (FR4) antibody (Alexa Fluor 647)

Antigen: Folate Receptor 4 (FR4)

Clonality: Monoclonal (12A5)

Host: Rat

Reactivity: Mouse (Murine)

Conjugate: Alexa Fluor 647

Application: Flow Cytometry (FACS)

Catalog no.: ABIN311967

Additional Information

Alternative name: FR4 (Folate Receptor 4)

Isotype: IgG2b kappa

Clone: 12A5

Description: Folate receptor 4 (FR4) is a surface receptor for folic acid (Vitamin B9). It was recently reported that FR4 is highlyand constitutively expressed on mouse CD4+ CD25+ natural regulatory T cells (Treg). The high expression of thissurface marker on Treg can be used in combination with CD4 and CD25 to distinguish Treg from other types of Tcells. Since FR4 is a surface marker, this antibody may be used in combination with anti-CD4 and/or CD25 to sortliving T reg cells for functional studies.

Application Details

Application Notes: Each lot of this antibody is quality control tested by immunofluorescentstaining with flow cytometric analysis. For immunofluorescent staining, thesuggested use of this reagent is approx. 0.25 ug per 106 cells in 100 ul volume. It isrecommended that the reagent be titrated for optimal performance for otherapplications.* Alexa Fluor

Purification: The antibody was purified by affinity chromatography, and conjugated withAlexa Fluor 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor 647.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Storage: The antibody solution should be stored undiluted at 4 degrees C and protected from prolonged exposure to light. Do not freeze.

Restrictions: For Research Use only