Notch 3 (NOTCH3) antibody (Alexa Fluor 647)

Antigen: Notch 3 (NOTCH3)

Synonyms: CASIL, CADASIL

Clonality: Monoclonal (HMN3-133)

Host: Hamster

Reactivity: Mouse (Murine)

Conjugate: Alexa Fluor 647

Application: Flow Cytometry (FACS)

Catalog no.: ABIN311990

Additional Information

Alternative name: Notch 3

Immunogen: Notch 3-Fc fusion protein

Isotype: IgG

Clone: HMN3-133

Description: The Notch receptors are highly conserved from invertebrates to mammals. While Notch 1 and Notch 2 exhibit thehighest structural similarity among the four mammalian Notch receptors. Notch 3 has a number of structural andfunctional differences. The binding of Notch 3 to its ligands results in the proteolysis of Notch and movement ofintracellular portions of Notch into the nucleus. This translocation triggers a series of signaling process. Notch 3 isprimarily expressed in adult arterial smooth muscle cells. Notch3 gene mutation can cause CADASIL, an inheritedearly stroke syndrome.

Application Details

Application Notes: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is approx. 1.0 ug per 106 cells in 100 ul volume. It isrecommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor

Purification: The antibody was purified by affinity chromatography, and conjugated withAlexa Fluor 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor 647.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Storage: The antibody solution should be stored undiluted at 4 degrees C and protected from prolonged exposure to light. Do not freeze.

Restrictions: For Research Use only