Add to Basket
Order hotline:
+1 404 474 4654
+1 888 205 9894 (TF)

T-Cell Surface Glycoprotein CD4 (CD4) antibody (APC/Cy7)

Antigen

T-Cell Surface Glycoprotein CD4 (CD4)
Synonyms CD4mut, L3T4, Ly-4, p55, W3/25
Clonality Monoclonal (W3-25)
Host
Alternatives

Mouse
Reactivity
Alternatives

Rat (Rattus)
Conjugate
Alternatives APC/Cy7
Application
Alternatives Flow Cytometry (FACS)
Catalog no. ABIN312137
Quantity 25 ug  (Variants)
Price Product not available in this region.
Shipping to

Additional Information
Application Details Alternatives Variants back to top

Alternative name CD4
Immunogen Rat thymocyte membrane glycoproteins
Isotype IgG1, kappa
Clone W3-25
Description CD4 is a 55 kD glycoprotein also known as T4. Rat CD4 is a member of the immunoglobulin superfamily and isexpressed on majority of thymocytes, macrophages, and a peripheral T cell subset (T helper cells). CD4 is a T cellco-receptor that interacts with the MHC class II molecule and is involved in T cell activation. The W3/25 antibody hasbeen shown to inhibit IL-2 production by T helper cells and to prevent autoimmune T cell transfer in an MBP inducedEAE model in vivo.

Application Details
Alternatives Variants back to top

Application Notes Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is approx. 0.5 ug per 106 cells in 100 ul volume. It isrecommended that the reagent be titrated for optimal performance for each application. Additional reported applications (for the relevant formats) include: immunohistochemistry1,2 of acetone-fixed frozensections, inhibition of IL-2 production3, inhibition of MBP-induced T cell activation in EAE transfer model3.
Purification The antibody was purified by affinity chromatography, and conjugated withAPC/Cy7 under optimal conditions. The solution is free of unconjugatedAPC/Cy7 and unconjugated antibody.
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Storage The antibody solution should be stored undiluted at 4 degrees C and protected from prolonged exposure to light. Do not freeze.
Restrictions For Research Use only

Alternatives
Application Details Variants back to top

Alternatives for antigen "T-Cell Surface Glycoprotein CD4 (CD4)", type "Antibodies"
Hosts Mouse (420), Rat (214), Rabbit (52)
Reactivities Human (292), Mouse (Murine) (230), Rat (Rattus) (102), Dog (Canine) (43), Chicken (34), Cow (Bovine) (33), Sheep (Ovine) (23), Pig (Porcine) (21), Cat (Feline) (20), Rabbit (20), Bird (Avian) (8), Guinea Pig (8), Monkey (4), Duck (3), Horse (Equine) (3)
Applications Flow Cytometry (FACS) (591), Immunohistochemistry (Frozen Sections) (IHC (fro)) (156), Immunoprecipitation (IP) (95), Western Blotting (WB) (57), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (54), Immunofluorescence (IF) (39), ELISA (36), Functional Studies (Func) (35), Immunohistochemistry (IHC) (26), Blocking Antibody (Inhibition) (14), Cytotoxicity Test (CyTox) (11), Immunocytochemistry (ICC) (11), Enzyme Immunoassay (EIA) (9), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Immunoelectron Microscopy (EM) (2), ELISA (Detection) (1), Neutralization (Neut) (1)
Conjugates FITC (109), Biotin (67), PE (43), APC (39), RPE (22), Cy5 (17), Cy7 (8), Cy5.5 (6), Pacific Blue (4), Alexa Fluor 488 (3), PE,Cy5 (3), PE,Cy7 (3), PerCP (3), SPRD (3), APC/Cy7 (2), Alexa Flour 488 (2), Alexa Fluor 700 (2), Cy3 (2), Gold (2), HRP (2), PE,Cy3 (2), PE/Cy7 (2), RBITC (2), APC,Cy7 (1), DY647 (1), Dyomics 647 (1), PE,Cy5.5 (1), PE,DY590 (1), PE,DY647 (1), PE-Dyomics 590 (1), PE-Dyomics 647 (1), PE/Cy5.5 (1), PerCP,Cy5.5 (1), RPE,Cy5 (1), RPE,Cy7 (1)
Epitopes domain 1 (10), domain 2 (5), non-polymorphic (5)